T7 RNA Poymerase (Uxinzelelo oluphezulu)
Inkcazo
I-T7 RNA Polymerase yi-DNA exhomekeke kwi-RNA-olymerase esuka kwi-T7 phage, enomsebenzi oqinileyo nothe ngqo we-5 ´→ 3 ´ RNA polymerase.I-T7 RNA Poplymerase ine-specific ephezulu yolandelelwano lomgqugquzeli we-T7 kwaye iya kudibanisa ubuninzi be-RNA ukusuka kwiqhekeza le-DNA elifakwe ezantsi ukusuka kumgqugquzeli.
Ulwakhiwo lweMichiza
Inkcazelo yeyunithi
Iyunithi enye ichazwa njengobungakanani be-enzyme efunekayo ukufaka i-1 nmol ATP kwi-acid-insoluble material kwi-1 iyure kwi-37 ° C.
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Iprotheyini enyulu | ≥95% |
| I-Nickase | Akukho nanye ifunyenweyo |
| Rnase | Akukho nanye ifunyenweyo |
| I-Endonuclease | Akukho nanye ifunyenweyo |
| I-Exonuclease | Akukho nanye ifunyenweyo |
| Ukucaciswa koMkhuthazi | Ayibonakali kwimveliso engachazwanga |
| E.coli gDNA | ≤0.1 pg/50U |
| Endotoxin | Uvavanyo lwe-LAL, ≤10EU/mg |
Ukuthutha kunye nokugcinwa
Ezothutho:ngaphantsi kwe-0 °C
Ugcino:Onke amacandelo kufuneka agcinwe kwi -25～-15°C
Ubomi obucetyiswayo bokuvavanya kwakhona:Iminyaka emi-2
iimveliso ezinxulumene
Bhala umyalezo wakho apha kwaye uwuthumele kuthi
