I-Ultra Nuclease

Inombolo yeCas: 9025-65-4

EC.No.: 3.1.30.2

Iphakheji: 20μL, 200μL, 2mL, 20mL

Thumela uMbuzo

Iinkcukacha zeMveliso

Inkcazo

I-UltraNuclease yi-endonuclaese eyenziwe ngofuzo evela kwi-Serratia marcescens, ekwazi ukuthobisa i-DNA okanye i-RNA, i-double okanye i-single stranded, i-linear okanye i-circular phantsi koluhlu olubanzi lweemeko, ihlaze ngokupheleleyo i-nucleic acids kwi-5'-monophosphate oligonucleotides kunye ne-3-5. ubude besiseko.

Emva kokuguqulwa kobunjineli bezofuzo, imveliso yabiliswa, yachazwa, kwaye yahlanjululwa kwi-Escherichia coli (E. coli), enciphisa i-viscosity ye-cell supernatant kunye ne-cell lysate kuphando lwezenzululwazi, kodwa iphucula ukusulungekiswa kobuchule kunye nophando olusebenzayo lweprotheni.Isenokusetyenziswa kunyango lwemfuza, ukuhlanjululwa kwentsholongwane, ukuveliswa kwesitofu sokugonya, iprotheyini kunye ne-polysaccharide ishishini lamachiza njenge-reagent ye-residue ye-nucleic acid yokususwa.

Ulwakhiwo lweMichiza

Inkcazelo yeyunithi

Isixa se-enzyme esetyenziselwa ukutshintsha ixabiso lokufunxa △A260 nge-1.0 ngaphakathi kwe-30min kwi-37 °C, i-pH 8.0, ilingana ne-37μg ye-salmon yesidoda se-DNA ngokusikwa kwi-oligonucleotides, yachazwa njengeyunithi esebenzayo.

Ukusetyenziswa kunye nomthamo

• Susa i-nucleic acid yangaphandle kwiimveliso zokugonya, yehlisa umngcipheko wentsalela yetyhefu ye-nucleic acid kwaye uphucule ukhuseleko lwemveliso.
• Nciphisa i-viscosity yolwelo lwefidi olubangelwa yi-nucleic acid, nciphisa ixesha lokulungiswa kunye nokwandisa isivuno seprotheyini.
• Susa i-asidi ye-nucleic esongiweyo (intsholongwane, i-inclusion body, njl.njl.), enceda ukukhululwa kunye nokucocwa kwesuntswana.
• Unyango lwe-nuclease lunokuphucula isisombululo kunye nokubuyiswa kwesampulu yekholomu ye-chromatography, i-electrophoresis kunye nohlalutyo lwe-blotting.
• Kwi-gene yonyango, i-nucleic acid iyasuswa ukufumana iintsholongwane ezihlambulukileyo ezihambelana ne-adeno.

Inkcazo

Izinto zovavanyo	Iinkcukacha
Inkcazo	Icacile kwaye ayinaMbala
Umsebenzi	≥ 250 U/ul
Umsebenzi othile	≥1.1*10⁶U/mg
Ubunyulu (SDS-PAGE)	≥ 99.0%
IiProteases	Akukho nanye ifunyenweyo
Umthwalo wezinto eziphilayo	＜10 cfu/100,000U
Endotoxines(LAL-test)	＜ 0.25EU/1,000U