Ikhithi yovavanyo lwe-DNase (i-Fluorescence) Umfanekiso obonakalayo

Ikhithi yovavanyo ye-DNase (Fluorescence)

Inombolo yeCas: 9003-98-9

Ubunyulu.: 90%

Iphakheji: 48Test, 192Test

Thumela uMbuzo

Iinkcukacha zeMveliso

Inkcazo

Ikiti yokufumanisa i-DNase isekelwe kwi-fluorophore ebhalwe nge-DNA probe.Xa isampuli ingenayo umsebenzi we-DNase, i-probe izinzile kwaye ayivelisi isignali ye-fluorescent;xa isampulu iqulethe umsebenzi we-DNase, i-probe ithotywa, ikhokelela kwisignali ye-fluorescence eyandisiweyo;izinga lokunyuka kwesignali ye-fluorescence ihambelana kakuhle nenani kunye nomsebenzi we-enzymes.Sebenzisa i-fluorescence microplate reader ukulinganisa kwi-wavelength ye-ex/em=485/525nm ukufumanisa ukuba isampuli ingcolisekile yi-DNase

Ulwakhiwo lweMichiza

Ukwakhiwa kweKit

Igama	48Uvavanyo	192 Uvavanyo
I-10 × isisombululo sokuphendula	0.5mL	2.0mL
DNA probe	1 ityhubhu	1 ityhubhu
TE buffer	0.5mL	2.0mL
I-DNase I esemgangathweni (2U/μL)	10μL	20μL
Isithinteli sokuDilution esiMgangatho	6mL	12mL
I-DNase (amanzi angenayo i-RNase).	25mL	25mL
DNase RNase kude	50mL	50mL

Inkcazo

Izinto zovavanyo	Iinkcukacha
Imbonakalo	Gqibezela ukupakisha kwaye akukho kuvuza kwamanzi
Umda wokufunyanwa	DNase I: 1.25x10^-6U/μL
Ukuchaneka	I-Intra assay CV ≤ 10%