I-Fructosyl-peptide Oxidase (FPOX)
Inkcazo
I-enzyme iluncedo ekumiselweni kwe-fructosyl-peptide kunye ne-fructosyl-L-amino acid.
Ulwakhiwo lweMichiza
Umgaqo wokusabela
I-Fructosyl-peptide + H2O +O2→ Peptide + Glucosone + H2O2
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Umgubo omhlophe we-amorphous, u-lyophilized |
| Umsebenzi | ≥4U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| IsiCatalase | ≤0.01% |
| ATPase | ≤0.005% |
| IGlucose oxidase | ≤0.03% |
| ICholesterol oxidase | ≤0.003% |
Ukuthutha kunye nokugcinwa
Ezothutho: I-Ambient
Ugcino:Gcina kwi -20 ° C (Ixesha elide), 2-8 ° C (ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi:Iminyaka emi-2
Imbali yophuhliso
Esinye sezikhombisi ezisetyenziselwa ukuxilongwa kwesifo sikashukela yi-glycated hemoglobin (HbA1c).Umlinganiselo we-HbA1c usebenzisa i-enzymes ifanelekile ukusetyenzwa kwamanani amaninzi eesampuli, kwaye ineendleko ezifanelekileyo.Ngaloo ndlela, kudala kukho umnxeba oqinileyo ovela kubasebenzi bezempilo malunga nokuphuhliswa kovavanyo lwe-enzyme.Ke ngoko, siphuhlise uvavanyo olutsha sisebenzisa "indlela yedipeptide".Ngokukodwa, sifumene "i-Fructosyl-peptide Oxidase" (FPOX) enokusetyenziswa njenge-enzyme yolu vavanyo.Oku kuququzelele impumelelo yethu ekuphumezeni eyokuqala yehlabathi ngokwenza ubunyani bovavanyo lwe-enzyme ye-HbA1c.Le "ndlela ye-dipeptide" isebenzisa i-Protease (i-Proteolytic enzyme) ukuphula i-HbA1c egazini, kwaye emva koko ilinganisa amanqanaba e-dipeptides e-saccharified eveliswa ngokusebenzisa i-FPOX.Le ndlela idibene nokwamkelwa okuhle kakhulu ngenxa yokufaneleka kwayo ukuba ilula, ingabizi kwaye ikhawuleze, kwaye i-HbA1c yokulinganisa i-reagent esebenzisa i-FPOX ngoku sele isetyenziswe kwihlabathi lonke.
