Ialkaline Phosphatase(ALP)

I-Alkaline Phosphatase ithathwa kwi-recombinant E. coli strain ethwala i-TAB5 gene.I-enzyme ivuselela i-dephosphorylation ye-5' kunye ne-3' ekupheleni kwe-DNA kunye ne-RNA phosphomonoesters.Kwakhona, i-hydrolyses ribose, kunye ne-deoxyribonucleoside triphosphates (NTPs kunye ne-dNTPs).I-TAB5 i-Alkaline Phosphatase isebenza ku-5' ukuphumela ngaphandle, 5' ukuhla kunye neziphelo ezibuthuntu.I-Phosphatase ingasetyenziswa kwizicelo ezininzi zebhayoloji yemolekyuli, ezinje nge-cloning okanye ukuphonononga iilebhile zokuphelisa ukususa iziphelo ze-phosphorylated ze-DNA okanye i-RNA.Kwiimvavanyo ze-cloning, i-dephosphorylation ikhusela i-DNA ye-plasmid yomgca kwi-self-ligation.Isenokwehlisa umgangatho we-dNTPs ezingadityaniswanga kwiimpendulo ze-PCR ukulungiselela itemplate yokulandelelana kwe-DNA.I-enzyme ingasebenzi ngokupheleleyo kwaye ayinakuguqulwa ngokufudumeza kwi-70 ° C kwimizuzu emi-5, ngaloo ndlela ikhupha i-phosphatase ngaphambi kokubopha okanye ukuphelisa ukuleyibhile ngokungeyomfuneko.