Ikhithi yovavanyo lwe-RNase (Fluorescence)
Inkcazo
Ikiti yokufumanisa i-RNase isekelwe kwi-fluorophore ebhalwe nge-RNA probe, eyenza abaphandi balinganise umsebenzi we-RNase.Xa isampuli ingenayo umsebenzi we-RNase, i-probe izinzile kwaye ayivelisi isignali ye-fluorescent;xa isampulu iqulethe umsebenzi we-RNase, i-probe ithotywa, ikhokelela kwisignali ye-fluorescence eyandisiweyo;Izinga lokunyuka kwesignali ye-fluorescence ihambelana kakuhle nenani kunye nomsebenzi we-enzymes.
Ulwakhiwo lweMichiza
Ukwakhiwa kweKit
Igama | 48Uvavanyo | 192 Uvavanyo |
I-10 × isisombululo sokuphendula | 0.5mL | 2.0mL |
RNA probe | 1 ityhubhu | 1 ityhubhu |
TE buffer | 0.5mL | 2.0mL |
I-RNase A esemgangathweni (10mg/mL) | 10μL | 20μL |
Isithinteli sokuDilution esiMgangatho | 6mL | 12mL |
I-DNase (amanzi angenayo i-RNase). | 25mL | 25mL |
DNase RNase kude | 50mL | 50mL |
Inkcazo
Izinto zovavanyo | Iinkcukacha |
Imbonakalo | Gqibezela ukupakisha kwaye akukho kuvuza kwamanzi |
Umda wokufunyanwa | RNase A: 0.313pg/mL |
Ukuchaneka | I-Intra assay CV ≤ 10% |
Ukuthutha kunye nokugcinwa
Ezothutho:Umkhenkce owomileyo
Ugcino:Gcina ku -20°C(kuphephe ukukhenkceza okuphindaphindiweyo nokunyibilika)
Ubomi obucetyiswayo bokuvavanya kwakhona:1 unyaka
iimveliso ezinxulumene
Bhala umyalezo wakho apha kwaye uwuthumele kuthi