T4 RNA ligase
Inkcazo
Le mveliso ixhomekeke kwi-ATP ye-T4 RNA ligase (T4 RNA Ligase I) iphinda ichazwe ngu-E. coli.Le enzyme ibangela ukubunjwa kweebhondi ze-phosphodiester phakathi kwe-oligonucleotides, i-RNA ene-single-stranded kunye ne-DNA intermolecular / intra-molecular 5'-PO4 kunye ne-3'-OH.
Inkcazelo yeyunithi
Iyunithi enye ichazwa njengobungakanani be-enzyme efunekayo ukuguqula i-nanomole e-1 ye-5´-[³²P] rA16 ibe yifomu echasene ne-phosphatase kwimizuzu engama-30 kwi-37°C.
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Umsebenzi we-Enzyme | 10 U/μL |
| Umsebenzi weProtheyini | ≥ 95% |
| I-Nickase | Ayibonakali |
| Umsebenzi we-RNase | Ayibonakali |
| Ecoli gDNA | ≤1ikopi/50U |
| Endotoxin | LCL-Test,≤10EU/mg |
Ukuthutha kunye nokugcinwa
Ezothutho:Umkhenkce owomileyo
Ugcino:Gcina ku -25~-15°C(kuphephe ukukhenkceza okuphindaphindiweyo kunye nokunyibilika)
Ubomi obucetyiswayo bokuvavanya kwakhona:Iminyaka emi-2
iimveliso ezinxulumene
Bhala umyalezo wakho apha kwaye uwuthumele kuthi
