BspQI Uthintelo Endonuclease
Inkcazo
I-BspQI inokuphinda ibonakaliswe kwi-E. coli ekwazi ukuqaphela iindawo ezithile kwaye iveliswe phantsi kwemigangatho ye-GMP.Isetyenziswa ngokubanzi kwimephu yemfuza, i-cloning kunye neminye imimandla.Le enzyme inqumla ngokukhawuleza i-DNA ukwenzela umgca wemfuza osebenzayo.
5'·······GGTC(N)1······3'
3'······CCAGAG(N)4·······5'
Ulwakhiwo lweMichiza
Inkcazelo yeyunithi
Iyunithi enye ichazwa njengobungakanani be-enzyme efunekayo ukugaywa kwe-1 µg ye-λ DNA ngeyure eyi-1 kwi-50 ° C kwi-total reaction volume ye-50 µl.
Iimpawu zeMveliso
• I-endonuclease yesithintelo inomsebenzi ophezulu we-enzymatic kwaye igqibezela ukugaya ngokukhawuleza.
• Umsebenzi osezantsi we-endonuclease ongangqalanga ukuqinisekisa ukusika okuchanekileyo "kwe-scalpel".
• Akukho nkqubo ye-BSA, ukunciphisa ungcoliseko lomthombo wobushushu.
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Iprotheyini enyulu | ＞98% |
| Iintsalela zeProtease | Akukho nanye ifunyenweyo |
| Uqhagamshelo kunye nokusika ukubonwa | Ukucoleka kokwetyisa ＞95% |
| DNase | Akukho nanye ifunyenweyo |
| Nicaknase | Akukho nanye ifunyenweyo |
Ukuthutha kunye nokugcinwa
Ezothutho:Ithunyelwe ngenqanawa ngaphantsi kwe-0 °C
Ugcino:Gcina kwi -20 °C
Ubomi obucetyiswayo bokuvavanya kwakhona:2years
