I-Ribonuclease III (RNase III)
Inkcazo
Le mveliso i-ribonuclease III (RNase III) iphinda ichazwe ngu-E. coli.Le exonuclease ekhethekileyo iqhekeza i-RNA enemisonto ephindwe kabini (dsRNA) kwaye ivelise i-12-35bp dsRNA amaqhekeza kunye ne-5'-PO4 kunye ne-3'-OH, i-3' overhangs.
Ulwakhiwo lweMichiza
Inkcazelo yeyunithi
Inkcazo yeyunithi yomsebenzi: Iyunithi enye ibhekisa kwisixa se-enzyme efunekayo ukuthoba i-1 μg ye
I-dsRNA ukuya kwi-siRNA kwindlela yokusabela ye-50 μL kwi-37 ° C imizuzu engama-20.
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Umsebenzi we-Exonuclease | Ukukhutshwa＜ 0.1% ye-radioactivity iyonke |
| Umsebenzi weDnase ongaKhethekanga | Ayibonakali |
| Uvavanyo lweProtein Purity Assay(SDS-PAGE) | ≥ 95% |
| Umsebenzi we-RNase (Ukwetyisa okwandisiweyo) | ＞90% ye-substrate ye-RNA ihlala injalo |
Ukuthutha kunye nokugcinwa
Ezothutho:Umkhenkce owomileyo
Ugcino:Gcina kwi -25~-15°C
Ubomi obucetyiswayo bokuvavanya kwakhona:Iminyaka emi-2
iimveliso ezinxulumene
Bhala umyalezo wakho apha kwaye uwuthumele kuthi
