BsaI Uthintelo Endonuclease Featured Umfanekiso

I-Endonuclease yesithintelo se-BsaI

Ubunyulu: 20U/μL

Iphakheji: 50μL, 250μL, 1ml, 10ml, 100ml

Thumela uMbuzo

Iinkcukacha zeMveliso

Inkcazo

I-enzyme yothintelo lwe-BsaI luhlobo lwe-II yesithintelo se-endonuclease ephuma kwiprotheyini ephinda iphinda ifakwe kwi-BspQI gene kwi-Bacillus sphaericus echazwe ngu-E.coli.Uthintelo lwe-endonucleases lusetyenziswa ngokubanzi kwimephu yemfuza, i-cloning kunye neminye imimandla.Le enzyme inqumla ngokukhawuleza i-DNA ye-gene ye-linearization esebenzayo i-Bsa I, i-IIs isithintelo se-endonuclease restriction endonuclease, iphuma kwi-recombinant E. coli strain ephethe i-BsaI edibeneyo kunye ne-modified gene esuka kwi-Bacillus stearothermophilus.ezineenkcukacha ezifanayo njengee-enzymes zomthonyama kunye nokunciphisa umsebenzi weenkwenkwezi.Ulandelelwano lokuqatshelwa kwayo kunye neendawo zokuqhawula zihamba ngolu hlobo lulandelayo:

5'·······GGTC(N)······· ··3'

3'······CCAGAG(NNNNN)·······5'

Ulwakhiwo lweMichiza

Iimpawu zemveliso

• I-endonuclease yesithintelo inomsebenzi ophezulu we-enzymatic kwaye igqibezela ukusabela kokugaya ngokukhawuleza.

• Umsebenzi osezantsi we-endonuclease ongangqalanga ukuqinisekisa ukusika okuchanekileyo "kwe-scalpel".

• Akukho nkqubo ye-BSA, ukunciphisa ungcoliseko lomthombo wobushushu.

Inkcazo

Izinto zovavanyo	Iinkcukacha
Umsebenzi we-Enzyme	20 U/µL
Ubunyulu(SDS-PAGE)	≥95%
Rnase	Akukho nanye ifunyenweyo
Umsebenzi we-DNase ongaKhethekanga	Ayifunyaniswanga
Star Umsebenzi	Ayifunyaniswanga
I-Ligation kunye nokuSikwa kwakhona	Emva kokugaya, amaqhekeza e-DNA anokudityaniswa.Kwaye la maqhekeza adibeneyo anokusikwa kwakhona nge-Bsal.
Endotoxins(LAL-test)	≤10 EU/mg
E.Coli DNA	≤1 Ikopi/2U

Ulawulo lwemeko

Ubunyulu ≥ 95%, Akukho DNase, umsebenzi we-RNase, i-Host DNA residue ≤ 100pg / mg, i-protein ye-host ≤ -50ppm, i-endotoxin eseleyo ≤ 10EU / mg, Akukho msebenzi we-protease, oyinyumba, ongenayo i-mycoplasma

Isicelo

Ukwetyisa iplasmid ukulungiselela isiqwenga seDNA esinemigca ekupheleni kwePoly (A/T/C/G);

Yenza umgca wetemplate yeplasmid phambi kokukhutshelwa kwe-in vitro;

Ukuncitshiswa kwentlupheko;

Inkcazelo yeyunithi

Iyunithi enye ichazwa njengobungakanani be-enzyme efunekayo ukwetyisa i-1 µg ye-DNA yolawulo lwangaphakathi kwiyure e-1 kwi-37 ° C kumthamo opheleleyo wokusabela we-50 µL.