Hs Taq DNA Polymerase(Glycerol-free) Umfanekiso okhoyo

I-Hs Taq DNA Polymerase(iGlycerol-free)

Inombolo ye-CAS: 9012-90-2 EC inombolo: 2.7.7.7

Iphakheji: 1000U 5000U 50000U

Thumela uMbuzo

Ingcaciso yeMveliso

Iingenelo

Umsebenzi we-Taq enzyme unokukhululwa ngokupheleleyo ngokufudumeza kwi-95 ° C nge-30 s

Uvakalelo oluphezulu lokukhulisa kunye neenkcukacha

Iyahambelana neenkqubo ezahlukeneyo zePCR/qPCR

Inkcazo

Isiqalo esishushu iTaq DNA Polymerase(iGlycerol-free) sisilwanyane somntu oshushu-isiqalo iTaq DNA polymerase eyenzelwe ukuphuhlisa uvavanyo lwe-lyophilized.I-Taq DNA polymerase yi-enzyme ye-Taq eshushu efunyenwe ngokuxuba i-antibody ye-Taq kunye ne-Taq DNA polymerase kumlinganiselo othile.Ngokusekwe kuzinzo lwe-thermal ye-antibody ye-Taq, i-Taq DNA polymerase isenokugcina ukutywinwa okungqongqo kwi-55 ° C, ukwenzela ukuba ukukhulisa okungangqalanga kunokucinezelwa ukuya kwiqondo eliphantsi ngexesha lesampulu yokuxuba kunye nezigaba zokufudumeza.Xa ukusabela kugcinwa kwi-95 ° C ngaphezu kwe-30 sec, i-antibody ye-Taq ayisebenzi ngokupheleleyo, kwaye umsebenzi we-enzyme ye-Taq ukhululwe ngokupheleleyo, oqinisekisa ukuba inkqubo ye-PCR inovakalelo oluphezulu lokukhulisa kunye neenkcukacha.

Ubume beMichiza

Inkcazo

Izinto zovavanyo	Iziphumo
Iprotheyini ecocekileyo	≥95%
Ukuthintela Isiphumo	≥99%
Umsebenzi we-Exonuclease	Ayifunyaniswanga
Umsebenzi we-Nickase	Ayifunyaniswanga
Umsebenzi weRnase	Ayifunyaniswanga
Uvakalelo lokukhulisa	Dlula

Usetyenziso

I-Hs Taq DNA Polymerase Glycerol Free iphuhliselwe usetyenziso olubandakanya ukuzenzekelayo kunye nokomisa komkhenkce.Ukuqulunqwa kwayo kwasimahla kweGlycerol kukufanelekele usetyenziso lwePCR oluzenzekelayo, okanye apho ukufakwa kwemibhobho echanekileyo yemithamo emincinci kubalulekile.

Yintoni eyenziwa yiGlycerol

IGlycerol idla ngokuba yinxalenye enkulu yesithintelo sokugcina ii-enzymes, kwaye isebenza njenge-cryoprotectant.I-Glycerol iphazamisa ubume bamanzi kwaye yenza i-buffer ifana neseli, ngaloo ndlela izinzile i-polymerase.I-Glycerol i-liquid e-viscous kakhulu kwaye ngoko ke inzima kwaye ichitha ixesha kwipayipi ngokuchanekileyo, ngakumbi kwimiqulu emincinci.Ngenxa yoko, ukubhobhoza i-glycerol kwiinkqubo ezizisebenzelayo zerobhothi ezikhawulezayo ziphantse zibe ngumngeni ongasonjululwanga.Ngaphaya koko, ubukho be-glycerol kwi-enzyme buffer yenza ukuba umkhenkce ukoma kungenzeki.