Multiplex One Step RT-qPCR Probe Kit (UDG Plus)
Inkcazo
I-Multiplex One Step RT-qPCR Kit (UDG Plus) yiprobe-based RT-qPCR yokubona ngokukhawuleza ikiti ilungele i-single-plex okanye i-multi-plex quantitative PCR isebenzisa i-RNA njenge template (efana nentsholongwane ye-RNA).Isetyenziselwa ukukhulisa i-DNA ephindwe kabini ukusuka kwiitemplates ze-RNA ezinestranded ukuvumela ubungakanani bexesha lokwenyani elikhawulezayo leethagethi zeRNA.Kwinyathelo lokukhutshelwa umva i-reverse transcriptase idibanisa iimolekyuli zeDNA ezinestranded (cDNA) ezincedisana netemplate yeRNA.
Inkqubo yokusabela
Amacandelo
| Amacandelo | 200rxns | I-1,000rns | 10,000 rxns |
| RNase-free ddH2O | 3*1ml | 15ml | 150ml |
| 5*umxube wenyathelo elinye | 800μl | 4*1ml | 40ml |
| Inyathelo elinye lomxube we-enzyme | 200μl | 1ml | 10ml |
| 50* ROX reference Daye 1 | 80μl | 400μl | 4*1ml |
| 50* ROX isalathiso Idayi 2 | 80μl | 400μl | 4*1ml |
a.Isithinteli esineNqana-linye sibandakanya i-dNTP Mix kunye ne-Mg2+.
b.I-Enzyme Mix iqulethe ikakhulu i-reverse transcriptase, i-Hot Start Taq DNA polymerase (ukuguqulwa kwe-antibody) kunye ne-RNase inhibitor.
c.Isetyenziselwa ukulungisa impazamo yeesinal zefluorescene phakathi kwamaqula ahlukeneyo.
c.I-ROX: Kuya kufuneka ukhethe ulungelelwaniso ngokwemodeli yesixhobo sovavanyo.
Usetyenziso
Ukufunyanwa kweentsholongwane
Ukuxilongwa kunye nophando lwe-tumor
Ukufunyanwa kwezifo zezilwanyana
Ukuxilongwa kwangaphambili kwezifo zefa
Ukufunyanwa kwee-microorganisms ze-pathogenic ekudleni
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina kwi -30 ~ -15 ℃.
Ubomi beShief:1 iminyaka
