I-RT-LAMP Reagent (-20℃)
Inkcazo
I-LAMP okwangoku isetyenziswa ngokubanzi iteknoloji kwintsimi ye-isothermal amplification.Isebenzisa ii-primers ze-4-6 ezinokuthi zichonge imimandla ethile ye-6 kwi-gene ekujoliswe kuyo, kwaye ixhomekeke kumsebenzi oqinileyo wokufuduka kwe-strand ye-Bst DNA polymerase.Kukho iindlela ezininzi zokubona i-LAMP, kubandakanywa indlela yedayi, indlela ye-pH colorimetric, indlela ye-turbidity, i-HNB, i-calcein, njl njl. I-RT-LAMP yolunye uhlobo lwe-LAMP yokuphendula kunye ne-RNA njenge template.I-RT-LAMP Kit (i-UDG plus) iphuhliswe ngokusekelwe kwindlela ye-fluorescence kwaye iqulethe i-Bst DNA Polymerase, i-thermotolerant reverse transcriptase, i-murine RNase inhibitor, kunye ne-UDGase.Le khithi ye-LAMP inovakalelo oluphezulu, ukusetyenziswa kakuhle kwe-primer, kunye nokusebenza okugqwesileyo ngokukodwa, ukunyamezela, kunye nokuzinza.
Amacandelo
| 100T | 1000T |
| 600μL | 6mL |
| 100 μL | 1 ml |
1) I-Bst Buffer iqulethe i-dN (U) TP kunye ne-SYTO-9, njl., kunye ne-1×Bst Buffer sele iqulethe i-8 mmol/L Mg2+;
2) I-Bst Enzyme Mix (UDG plus) iqulethe i-UDGase, i-Reverse Transcriptase, i-Bst Ⅱ Polymerase, i-MRI;
Usetyenziso
I-LAMP reactions
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina kwi -20 ℃
Ubomi beShief:Iinyanga ezi-6
Izilumkiso
Ukhuseleko kunye nempilo yakho, nceda unxibe iidyasi zaselebhu kunye neeglavu ezilahlwayo xa usebenza.
