I-Trypsin edibeneyo
Inzuzo
1.Akukho mvelaphi yezilwanyana
2.Ukucoceka okuphezulu
3.Ulawulo lwenkqubo engcono
4.Umgangatho ozinzileyo
Inkcazo
I-Trypsin iqhawula ngokukodwa i-C-terminal peptide bonds ye-lysine kunye ne-arginine, enokuthi ithobe iiprotheni ezibophelelayo ze-intercellular.Ulandelelwano lwe-amino acid ye-trypsin ephinda-phindayo eveliswe yi-Hzymes Biotech iyafana naleyo ye-porcine pancreas-derived trypsin, kwaye iveliswa yi-recombinant Escherichia coli expression.Ingathatha indawo yotsalo lwemveli lwe-trypsin yokugaya iiseli kwizitofu zokugonya, iiseli ze-stem, unyango lwe-immune cell cell, ukuhlolwa kweziyobisi, amajoni omzimba kunye namanye amacandelo.I-Aprotinin, i-trypsin inhibitor yesoya, njl njl.
I-recombinant trypsin ineempawu ezifanayo njenge-trypsin ekhutshwe ngokwendalo.Le mveliso yenziwa kwiworkshop yebakala leGMP.Akukho mveliso yezilwanyana imvelaphi ekrwada isetyenziselwa ukubilisa, ukuhlanjululwa kunye nezithako zokugqibela.Ngexesha elifanayo, ukuveliswa kunye neenkqubo zokulawula umgangatho ziqinisekisiwe, okubangelwa izinga eliphezulu lokuhambelana phakathi kweebhetshi.
Ulwakhiwo lweMichiza
Inkcazelo yeyunithi
25℃, pH 7.6, 3.2ml impendulo yesisombululo(1cm indlela elula), iyunithi enye yetrypsin (U) yachazwa njenge
ukwanda kwe-0.003 kwixabiso lokufunxa kwi-253nm nge-enzymatic hydrolysis ye-BAEE ngomzuzu.
Ukusetyenziswa kweMveliso
Kwinkcubeko yeseli
• Ukwetyisa ibhloko yezicubu, ukufumana iiseli eziphambili.
• Ukwetyisa kweeseli ezibambeneyo.
• Ukukhuliswa kweeseli ngendlela ye-microcarrier.
• Ukwetyisa ngobunono iiseli ze-stem.
• Unyango lwe-immune cell, njl.
Kwiprotheyini edibeneyo
• Ukuveliswa kwakhona kwe-insulin.
• Ulandelelwano lweprotheyini, imephu yepeptide.
• Iinkqubo ezithile zeproteolytic ezifana nophando lweproteomics
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Imbonakalo | Umgubo omhlophe okanye omhlophe |
| Ukunyibilika (10mg/ml) | Sula isisombululo esingenambala |
| Ubunyulu | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Umsebenzi othile (Umgubo) | ≥2500U/mg |
| E.Coli intsalela yeprotheyini yokusingatha | ≤0.01% |
| Endotoxins(LAL-Test) | ≤20 EU/mg |
Ukuthutha kunye nokugcinwa
Ezothutho:Ithunyelwe ngaphantsi kwe-2-8 °C
Ugcino:Gcina kwi -2-8 ° C, emva kokuchithwa kunye ne-1mM hcl okanye i-50nM HAC, kufuneka igcinwe phantsi kwe -25 ~ -15 °C
Ubomi obucetyiswayo bokuvavanya kwakhona:Iminyaka emi-2
