Uricase(UA-R) evela kwiMicroorganism
Inkcazo
Le enzyme iluncedo kwi-enzymatic determi nation ye-uric acid kuhlalutyo lwezonyango.I-Uricase ithatha inxaxheba kwi-purine catabolism.Idala ukuguqulwa kwe-uric acid enganyibiliki kakhulu kwi-5-hydroxyisourate.Ukuqokelelana kwe-uric acid kubangela ukonakala kwesibindi/izintso okanye kubangele igawuthi ngokungapheliyo.Kwiimpuku, uguquko kwijini yokufaka ikhowudi ye-uricase kubangela ukwanda ngesiquphe kwe-uric acid.Iimpuku, ezinqongopheleyo kule gene, zibonisa i-hyperuricemia, i-hyperuricosuria, kunye ne-uric acid crystalline obstructive nephropathy.
Ulwakhiwo lweMichiza
Umgaqo wokusabela
Uric acid+O2+2H2O→ Allantoin + CO2+ H2O2
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Umgubo omhlophe we-amorphous, u-lyophilized |
| Umsebenzi | ≥20U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg umgubo/ml) | Cacileyo |
| Ukudibanisa ii-enzymes | |
| I-NADH/NADPH oxidase | ≤0.01% |
| IsiCatalase | ≤0.03% |
Ukuthutha kunye nokugcinwa
Ezothutho:Ithunyelwe ngaphantsi kwe-20°C
Ugcino :Gcina kwi -20°C(Ixesha elide), 2-8°C(Ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi:Iminyaka emi-2
iimveliso ezinxulumene
Bhala umyalezo wakho apha kwaye uwuthumele kuthi
