I-Hotstart Taq DNA Polymerase (5u/ul)
I-Taq DNA Polymerase sisiqalo esishushu se-DNA polymerase ngokuvalelwa kabini ngama-antibodies aphindwe kabini.Le mveliso ayithinteli kuphela i-5′→3′ polymerase umsebenzi we-Taq DNA polymerase, kodwa iphinda ithintele umsebenzi we-5′→3′exonuclease.Ukufudumeza imizuzwana engama-30 kwiqondo lokushisa langaphambi kwe-denaturation kunokuphelisa ngokupheleleyo i-antibody kunye nokukhulula umsebenzi we-DNA polymerase kunye nomsebenzi we-exonuclease.Uphawu lokuthintela kabini alunakukuthintela kuphela ukukhulisa okungachazwanga okubangelwa kukungahambelani okanye i-primer dimer, kodwa ikwanqanda ngokufanelekileyo ukuhla komqondiso we-fluorescence okubangelwa kukuthotywa kwe-probe, ukwenzela ukuba i-in vitro yokufunyaniswa kwe-reagent izinze ngakumbi ngexesha lokuthuthwa okanye ukusetyenziswa kwigumbi. ubushushu.
Amacandelo
| Icandelo | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μL | 200 μL | 2 ml | 5 ml |
Imeko yoGcino
Imveliso ithunyelwa ngeqhwa elomileyo kwaye ingagcinwa kwi -25 ° C ~ -15 ° C iminyaka eyi-2.
Iinkcukacha
| Ipolymerase | Taq DNA Polymerase |
| Ubunyulu | ≥ 95% (SDS-PAGE) |
| Isiqalo esishushu | Isiqalo esishushu esakhelwe ngaphakathi |
| Isantya sokuphendula | Umgangatho |
| Umsebenzi we-Exonuclease | 5′→3′ |
Imiyalelo
Ukuseta iRection
| Amacandelo | Umthamo (μL) | Ukugxininiswa kokugqibela |
| 2× Isithintelia | 25 | 1× |
| I-Primer/Probe mixb | × | 0.1 μmol/L-0.5 μmol/L |
| I-Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Isakhelo seDNAc | × | 0.1-100 ng |
| ddH2O | Ukuya kuthi ga kwi-50 | - |
Amanqaku:
1) Ngokwesicelo esithile sovavanyo, kuyafuneka ukulungiselela isithinteli sokusabela esihambelanayo.
I-2) Ubungakanani be-DNA kunye noxinzelelo lweeprobes okanye i-primers zinconywa ukugxininiswa.Ugxininiso olufanelekileyo lunokulungelelaniswa ngokwemiqathango ethile yovavanyo.
Iprothokholi yebhayisekile eshushu
| Inyathelo | Ubushushu(°C) | Ixesha | Imijikelo |
| I-denaturation yangaphambili | 95 ℃ | 5 imiz | 1 |
| I-Denaturation | 95 ℃ | 15 imizuzwana | 45 |
| Ukwandiswa / Ukwandiswa | 60 ℃a | 30 imizuzwanab |
Amanqaku:
I-1) Iqondo lokushisa lokuphendula lilungelelaniswa ngokwexabiso le-Tm yee-primers ezilungiselelwe.
2) Izixhobo ezahlukeneyo ze-qPCR zidinga ixesha lokufunyanwa kweempawu ze-fluorescence ezahlukeneyo, nceda usete ngokwelona xesha lifutshane.
Amanqaku
Nceda unxibe iPPE eyimfuneko, idyasi yelebhu kunye neeglavu, ukuqinisekisa impilo nokhuseleko lwakho!
