Uracil DNA Glycosylase (UDG/UNG)
Inkcazo
I-Heat-Labile UDG (i-uracil DNA glycosylase) ibangela ukukhululwa kwe-uracil yamahhala kwi-DNA ene-uracil nge-hydrolyzing i-N-glycoside bond phakathi kweziseko ze-uracil kunye ne-sugar-phosphate skeletons.Xa kuthelekiswa ne-enzymes ye-UDG eqhelekileyo, ukushisa-Labile UDG kunokuphepha ukuthotywa kwemveliso yokukhulisa i-dU equkethe i-dU ebangelwa ngumsebenzi oseleyo we-UDG engasebenziyo kwindawo yokushisa.I-Heat-Labile UDG (uracil DNA glycosylase) isebenza kwindawo yokushisa kunye ne-thermolabile kwaye kulula ukuba ingasebenzi.
Ulwakhiwo lweMichiza
Inkcazo
| I-enzyme | Glycosylase |
| Isithinteli esihambelanayo | Isithinteli sokuGcina |
| Ukushisa Ukungasebenzi | 50°C, 10 min |
| Inkcazelo yeyunithi | Iyunithi enye (U) ichazwa njengobungakanani be-enzyme efunekayo ukuze i-catalyze i-hydrolysis ye-1 μg dU equlethe i-dsDNA kwimizuzu engama-30 kwi-25 ° C. |
Usetyenziso
I-1.Isifundo sokulungiswa kwe-DNA kunye nokufumanisa ukuguqulwa kwenguqu.
2.Ukwandisa ukusebenza kakuhle kwe-cloning yeemveliso ze-PCR.
3.Ukwandisa ukusebenza kakuhle kwe-mutagenesis ejoliswe kwindawo.
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina ku -15 ℃ ~ -25 ℃
Ubomi beShief:iminyaka eyi-2
