Multiplex PCR Taq DNA Polymerase

I-Multiplex PCR DNA Polymerase sisizukulwana esitsha se-polymerase ye-DNA eshushu esekelwe kukuguqulwa kwe-antibody kwaye iphuculwe ukuphucula ubudlelwane betemplate.Inguqulelo yovakalelo oluPhezulu lwenziwe ngononophelo lwe-multiplex PCR enekhono lokukhulisa amandla kunye nokuqonda okuphezulu.Ngenkqubo ye-buffer efanelekileyo ye-multiplex PCR, iyahambelana noluhlu olubanzi lwemveliso ye-GC umxholo kunye nexabiso le-primer Tm.Uninzi lweemvavanyo ze-PCR ezininzi zinokwenziwa kwiqondo lobushushu le-annealing jikelele le-60°C ngaphandle kokulungiswa okongeziweyo.

Le mveliso inomgangatho ophezulu kakhulu wokukhulisa amandla, kwaye ngaxeshanye inokwandisa amaqhekeza ekujoliswe kuwo kuluhlu lwe-50 - 3,500 bp;inamandla okukhulisa amandla, kwaye inokwenza ukutyetyiswa kwamakhulu aliqela nangaphezulu;ukunyamezela ukungcola okugqwesileyo, ukuxhathisa kakhulu kwiindidi ze-inhibitors kunye neentlobo zokungcola, kwaye zihambelana nokukhulisa ngokuthe ngqo igazi elipheleleyo, amakhadi egazi, njl.