I-RT-LAMP Fluorescent Master Mix (i-Lyophilized Powder) Umfanekiso obonakalayo

I-RT-LAMP Fluorescent Master Mix (iPowder eLyophilized)

Inombolo yeCAS.:

Inombolo yeEC:

Iphakheji: 96T, 960T, 9600T.

Thumela uMbuzo

Iinkcukacha zeMveliso

Ingcaciso entsha

Ingcaciso yeMveliso

I-LAMP okwangoku isetyenziswa ngokubanzi iteknoloji kwintsimi ye-isothermal amplification.Isebenzisa ii-primers ze-4-6 ezinokuthi zichonge imimandla ethile ye-6 kwi-gene ekujoliswe kuyo, kwaye ixhomekeke kumsebenzi oqinileyo wokufuduka kwe-strand ye-Bst DNA polymerase.Kukho iindlela ezininzi zokubona i-LAMP, kubandakanywa indlela yedayi, indlela ye-pH colorimetric, indlela ye-turbidity, i-HNB, i-calcein, njl njl. I-RT-LAMP yolunye uhlobo lwe-LAMP yokuphendula kunye ne-RNA njenge template.I-RT-LAMP Fluorescent Master Mix (i-Lyophilized Powder) ikwimo ye-Lyophilized Powder, kwaye idinga kuphela ukongeza iiprimers kunye neetemplates xa usebenzisa.

Inkcazo

Izinto zovavanyo	Iinkcukacha
Endonulease	Akukho icinyiweyo
Umsebenzi we-RNase	Akukho nanye ifunyenweyo
Umsebenzi we-DNase	Akukho nanye ifunyenweyo
Umsebenzi we-Nickase	Akukho nanye ifunyenweyo
E. coli.gDNA	≤10iikopi/500U

Amacandelo

Le mveliso iqulethe i-Reaction Buffer, i-RT-Enzymes Mix ye-Bst DNA Polymerase kunye ne-Thermostable Reverse Transcriptase, i-Lyoprotectant kunye ne-Fluorescent Dye Components.