Proteinase K( Ulwelo)
Iingenelo
● Uzinzo oluphezulu kunye nomsebenzi we-enzyme esekelwe kubuchwepheshe obuchanekileyo be-evolution
● Iyanyamezela ityuwa yeGuanidine
● I-RNase ikhululekile, i-DNase ikhululekile kunye ne-Nickase yamahhala, i-DNA <5 pg/mg
Inkcazo
I-Proteinase K yi-serine protease ezinzileyo ene-substrate ebanzi.Ithoba iiproteni ezininzi kwilizwe lasekuhlaleni naxa kukho izinto zokucoca.Ubungqina obuvela kwikristale kunye nezifundo ze-molecular structure zibonisa ukuba i-enzyme yeyentsapho ye-subtilisin enendawo esebenzayo ye-catalytic triad (Asp 39-His 69-Ser 224).Eyona ndawo iphambili yokuqhekeka yi-peptide bond ekufuphi neqela le-carboxyl le-aliphatic kunye ne-amino acid enuka kamnandi enamaqela e-alpha amino avaliweyo.Ngokuqhelekileyo isetyenziselwa ukucaciswa kwayo okubanzi.
Ubume beMichiza
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Imbonakalo(Umbala) | Ayinambala ukuya kwiBrown yokuKhanya |
| INkangeleko( Turbidity) | Icacile ukuya kuHazy Kakhulu Kancinci |
| Inkangeleko (Ifomu) | Ulwelo |
| Umsebenzi | ≥800U/ml |
| Iprotheni mg protein / ml | ≥20 mg / ml |
| RNase | Akukho nanye ifunyenweyo |
| DNase | Akukho nanye ifunyenweyo |
| Nicase | Akukho nanye ifunyenweyo |
Usetyenziso
Ikhithi yokuxilongwa kwemfuzo;
I-RNA kunye ne-DNA extraction kits;
Ukukhutshwa kwezinto ezingezizo iiprotheyini ezivela kwizicubu, ukuthotywa kokungcola kweeprotheni, ezifana
Ugonyo lwe-DNA kunye nokulungiswa kwe-heparin;
Ukulungiswa kwe-chromosome DNA nge-pulsed electrophoresis;
Iblothi yaseNtshona;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Ukuthumela ngenqanawa kunye noGcino
Ukuhambisa ngenqanawe:I-Ambient
Imiqathango yoGcino:Gcina ku -20℃(Ixesha elide)/ 2-8℃( Ixesha elifutshane)
Umhla wokuphinda ucetywe:iminyaka eyi-2
