I-Glycohemoglobin A1c (HbA1c) Ikhithi yoVavanyo
Iingenelo
● Ukuchaneka okuphezulu
● Ikhono elinamandla lokuchasana nokuphazamiseka
● Uzinzo oluhle
Ulwakhiwo lweMichiza
Usetyenziso
Uvavanyo lwe-in vitro lokumisela ubungakanani be-HbA1c yoxinaniso kwigazi elipheleleyo lomntu kwiinkqubo ze-photometric.I-HbA1c yimveliso ye-hemoglobin (Hb) evelisa ukusabela okucothayo kunye nokuqhubekayo kwe-non-enzymatic glycation phantsi kweglucose ephezulu yegazi.IGlucose iguqula i-hemoglobin ngokukodwa kwintsalela ye-n-terminal valine ukuze yenze i-glycated hemoglobin.Phantsi kweemeko eziqhelekileyo ze-physiological, ukuveliswa kweemveliso ze-non-enzymatic glycosylation reaction ngokuqinisekileyo zihambelana nokuxinana kwee-reactants.Kuba ukuxinana kwe-hemoglobin kuhlala kuzinzile, amanqanaba e-glycosylation axhomekeke ikakhulu kuxinzelelo lweglucose kwaye anxulumene nobude be-hemoglobin kunye nokuvezwa kweglucose.Ke ngoko, i-HbA1c sisilathisi esilungileyo kumndilili weswekile yegazi kwizigulana kwiinyanga ezi-2-3 zokugqibela.
Umgaqo
Ngaphantsi kwesenzo se-protease, i-n-terminal ye-β chain kwi-HbA1c iyanqunyulwa kwaye i-glycosylated dipeptides ikhutshwe.Kwi-reaction yokuqala, i-Hb concentration inokufumaneka ngokulinganisa ukuxhamla kwe-480 nm.Kwimpendulo yesibini, i-fructosyl peptide oxidase (FPOX) isebenza kwi-glycosylated dipeptides ukuvelisa i-hydrogen peroxide enokuthi isebenze kunye ne-chromogenic agents ukwenza i-absorbection kwi-660nm phambi kwe-peroxidase, ngoko ukuxinwa kwe-HbA1c kunokufumaneka ngokulinganisa ukufunxa 660nm.Ngokutsho kwe-HbA1c efunyenweyo kunye ne-Hb concentration, ipesenti ye-HbA1c (HbA1c%) ingabalwa.
Esebenzayo
I-Hitachi 7180/7170/7060/7600 i-automatic biochemical analyser,Abbot 16000,OLYMPUS AU640automatic biochemical analyser
Iireagents
| Amacandelo | Ugxininiso |
| I-Reagent 1(R1) | |
| Isithinteli esihle | 100mmol/L |
| I-PRK | 500KU/L |
| DA-67 | 10mmol/L |
| iiReagents 2 (R2) | |
| Isithinteli esihle | 100mmol/L |
| I-Fructosyl peptide oxidase | 50 KU/L |
| I-Reagent 3(R3) | |
| Isithinteli esihle | 100mmol/L |
Ukuthutha kunye nokugcinwa
Ezothutho:I-Ambient
UGcino kunye nozinzo:
Ukuya kuthi ga kumhla wokuphelelwa obonakalisiweyo kwileyibhile, xa igcinwe ingavulwanga kwi-2-8℃ kwaye ikhuselwe ekukhanyeni.Emva kokuba ivuliwe, ii-reagents zizinzile iintsuku ze-28 xa zifakwe kwi-analyzer okanye kwifriji.
Ukungcoliswa kwee-reagents kufuneka kuphetshwe.Musa ukukhenkcela ii-reagents.
Emva kokuba inyibilikisiwe, i-calibrator izinzile iintsuku ezili-15 kwi-2-8 ℃, ulawulo luzinzile iintsuku ezi-7 kwi-2-8 ℃, musa ukuqhwala.
Beka ubomi kwishelufa:1 unyaka
