Uracil DNA Glycoylase (iGlycerol-free)
Inkcazo
I-UDG ye-Thermosensitive (uracil-DNA glycosylase) inokubangela i-hydrolysis yesiseko se-uracil ye-DNA chain equkethe i-uracil kunye ne-N-glycosidic bond ye-backbone ye-sugar-phosphate ukukhulula i-uracil yamahhala.Xa kuthelekiswa nee-enzymes eziqhelekileyo ze-UDG, ii-enzymes ze-UDG ze-thermosensitive zinqanda umsebenzi onokwenzeka wokushiyeka we-enzymes yesiqhelo ye-UDG emva kokungasebenzi, ezinokuthoba iimveliso zokukhulisa i-dU kwindawo yobushushu begumbi.Le mveliso isebenza kwiqondo lobushushu begumbi kwaye inoburhalarhume beqondo lobushushu kwaye iyakwazi ukungasebenzi.
Ulwakhiwo lweMichiza
Inkcazo
| I-enzyme | Glycosylase |
| Isithinteli esihambelanayo | Isithinteli sokuGcina |
| Ukushisa Ukungasebenzi | 50°C, 10 min |
| Inkcazelo yeyunithi | Iyunithi enye (U) ichazwa njengobungakanani be-enzyme efunekayo ukuze i-catalyze i-hydrolysis ye-1 μg dU equlethe i-dsDNA kwimizuzu engama-30 kwi-25 ° C. |
Usetyenziso
Susa ungcoliseko lwe-aerosol yemveliso ye-PCR ene-dU.
Ukususwa kweziseko ze-uracil kwi-DNA enye okanye i-double-stranded DNA
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina ku -15 ℃ ~ -25 ℃
Ubomi beShief:1years
