IKit ye-Ultra Nuclease Assay (ELISA)
Inkcazo
Le khithi ye-Ultra Nuclease ELISA yisanti enokuthi i-ELISA yenziwe ngefomathi ye-microplate.Iisampulu ezinokuthi ziqulathe i-endonuclease zifukanywe kwimingxuma yepleyiti yemicrotiter ebiqatywe ngaphambili nge-anti-endonuclease ecociweyo yokulwa ukubamba i-antibody.Emva kokufakwa kunye nesinyathelo sokuhlamba apho amacandelo angabotshwanga asuswe, i-enzyme-conjugated, i-anti-endonuclease detector antibody iyongezwa.Iimpendulo ze-immunoloji zikhokelela ekwenziweni kwesandwich complex yesigaba esiqinileyo se-antibody-endonuclease-enzyme ebhalwe i-antibody.Emva kwesinyathelo sokugqibela sokuhlamba, isisombululo se-substrate songezwa kumaqula kwaye siphendule, okubangela ukuphuhliswa kombala.Uxinaniso lwamehlo lulinganiswa nge-photometric kwaye lulingana ne
Hlalutya ukugxininiswa okukhoyo emigodini.Uxinzelelo lwe-endonuclease kwiisampuli ezingaziwayo zingabalwa ngokusekelwe kwigophe eliqhelekileyo elihambelanayo.
Ulwakhiwo lweMichiza
Inkcazelo yeyunithi
Ubungakanani be-enzyme esetyenziselwa ukutshintsha ixabiso lokufunxa lika-△A260 nge-1.0 ngaphakathi kwe-30min
kwi-37 °C, i-pH 8.0, ilingana ne-37μg ye-salmon ye-salmon ye-DNA egayiweyo ngokusika kwi-oligonucleotides, yachazwa njengeyunithi esebenzayo.
Ukusetyenziswa kunye nomthamo
• Susa i-nucleic acid yangaphandle kwiimveliso zokugonya, yehlisa umngcipheko wentsalela yetyhefu ye-nucleic acid kwaye uphucule ukhuseleko lwemveliso.
• Nciphisa i-viscosity yolwelo lwefidi olubangelwa yi-nucleic acid, nciphisa ixesha lokulungiswa kunye nokwandisa isivuno seprotheyini.
• Susa i-asidi ye-nucleic esongiweyo (intsholongwane, i-inclusion body, njl.njl.), enceda ukukhululwa kunye nokucocwa kwesuntswana.
• Unyango lwe-nuclease lunokuphucula isisombululo kunye nokubuyiswa kwesampulu yekholomu ye-chromatography, i-electrophoresis kunye nohlalutyo lwe-blotting.
• Kwi-gene yonyango, i-nucleic acid iyasuswa ukufumana iintsholongwane ezihlambulukileyo ezihambelana ne-adeno.
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Umda osezantsi wokufunyanwa | 0.6 ng/mL |
| Umda osezantsi wobungakanani | 0.2 ng/mL |
| Ukuchaneka | I-Intra assay CV≤10% |
Ukuthutha kunye nokugcinwa
Ezothutho:Ithunyelwe ngenqanawa ngaphantsi kwe-0 °C
Ugcino:Gcina kwi-2-8 ° C, i-reagent evuliwe evulekileyo ezinzileyo kwiiveki ezi-6
Ubomi obucetyiswayo bokuvavanya kwakhona:1 unyaka
