Isampulu yokuKhupha iReagent
Isampulu yokukhutshwa kweReagent yeyeemeko zokuxilonga ze-molecular POCT.Kwiinkqubo ezimbini zokukhulisa ngokuthe ngqo i-LAMP kunye ne-PCR yokukhulisa ngokuthe ngqo, akukho mfuneko yokukhutshwa kwe-nucleic acid.I-lysate ekrwada yesampulu inokwandiswa ngokuthe ngqo, i-gene ekujoliswe kuyo inokufunyanwa ngokuchanekileyo, ixesha lokufumanisa isampuli linokunqunyulwa ngakumbi, elihambelana ngokugqibeleleyo neemfuno zesicelo se-POCT ye-molecular.Ifanelekile i-swabs yeempumlo, i-throat swabs kunye nezinye iintlobo zeesampuli.Iisampulu ezicutshungulwayo zingasetyenziselwa ngokuthe ngqo kwi-real-time fluorescence quantitative PCR okanye i-LAMP yokubona, kunye neziphumo ezifanayo njengeendlela eziqhelekileyo zokukhutshwa ziyakwazi ukufezekiswa ngaphandle kwemisebenzi enzima ye-nucleic acid extraction.
Iimeko zokuGcina
Ukuthutha kunye nokugcina kwiqondo lokushisa.
Ulawulo lwemeko
Ukufunyaniswa okusebenzayo - qPCR yobungakanani: Inkqubo ye-Sample Release Release ye-800μl yandiswa
kunye neekopi ezili-1000 zeNoveli yePseudovirus, isampulu enye yempumlo yokuswabha, ekhokelela kwigophe elifanayo lokukhulisa kunyeΔCt amaxabiso ngaphakathi ± 0.5 Ct.
Inkqubo yoMfuniselores
1. Thatha i-800 μl yeSampuli yokuKhupha iReagent kwaye usasaze isisombululo se-lysis kwi-1.5 mL yesampuli ye-tube
2. Thatha i-swab yeempumlo okanye i-swab yomqala kunye ne-swab;Inkqubo yesampuli ye-swab yeempumlo: thatha i-swab engcolileyo kwaye uyifake emathatheni, ngokukhawuleza uqhubele phambili malunga ne-1.5 cm ubunzulu, jikethe ngokukhawuleza amaxesha ama-4 ngokuchasene ne-mucosa yeempumlo ngaphezu kwemizuzwana eyi-15. , uze uphinde usebenze kwenye indawo yeempumlo kunye ne-swab ye-Swab yoMqala isampuli yenkqubo: thatha i-swab eyinyumba kwaye ngobumnene, usule ngokukhawuleza i-tonsils ye-pharyngeal kunye nodonga lwangasemva lwe-pharyngeal amaxesha ama-3.
3.Beka i-swab ngokukhawuleza kwi-tube yesampuli.Intloko ye-swab kufuneka ijikelezwe kwaye ixutywe kwisisombululo sokugcina ubuncinane imizuzwana ye-30 ukuqinisekisa ukuba isampuli ikhutshwe ngokupheleleyo kwi-tube yesampuli.
4. Ukufakwa kwiqondo lokushisa kwegumbi (20 ~ 25℃) kwi-1min, ukulungiswa kwe-lysis buffer kugqityiwe.
5. Zombini i-25μl inkqubo ye-RT-PCR kunye ne-RT-LAMP yayihambelana ne-10μl isixa sokongeza itemplate kwiimvavanyo zokubona.
Amanqaku
1. Ubuncinci besampulu ye-lysate ngqo ehambelana ne-swab enye inokulungiswa kwi-400μl, enokuthi ihlengahlengiswe ngokweemfuno zovavanyo.
2. Emva kokuba isampuli iqhutywe yi-reagent yokukhululwa kwesampuli, kucetyiswa ukuba kuqhutywe isigaba esilandelayo sovavanyo ngokukhawuleza, ixesha lokulinda lekhefu likhethwa ukuba lingaphantsi kweyure ye-1.
3. I-pH yesampuli ye-lysate i-acidic, kwaye inkqubo yokufumanisa kufuneka ibe ne-buffer ethile.Ifanelekile kuninzi lwe-PCR, i-RT-PCR, kunye ne-LAMP fluorescence ubhaqa kunye ne-pH buffer, kodwa ayifanelekanga ukufunyanwa kombala we-LAMP ngaphandle kwe-buffer.
