I-RT-LAMP Fluorescent (ibhola e-Lyophilized)
I-RT-LAMP Fluorescent Master Mix (Lyophilized Powder) iqulethe i-reaction buffer, i-Bst DNA polymerase kunye ne-heat-resistant reverse transcriptase, i-lyophilized protectant, kunye nedayi ye-fluorescent.Imveliso ikwimo yeentsimbi ze-lyophilized, kwaye kuphela ii-primers kunye neetemplates kufuneka zongezwe ukusetyenziswa.
iReagent Component
I-RT-LAMP Fluorescent Master Mix (iPowder eLyophilized)
Ukusetyenziswa
YeDNA okanye iRNA isothermal amplification
Iimeko zokuGcina
Ezothutho kunye nevenkile kwi-2~8°C.
Ubomi beshelufu: iinyanga ezili-12.
Umgaqo-nkqubo
1. Thatha inani elihambelanayo lamaso e-lyophilized ngokwenani leemvavanyo.
2. Ukulungiswa kwenkqubo yokusabela
| Icandelo | Umthamo |
| I-RT-LAMP Fluorescent Master Mix (Umgubo weLyophilized) | 2 amaso |
| Idayi yeFluorescent | x μL |
| 10 × Primer Mix a | 5 μL |
| Izakhelo zeDNA/RNA b | n μL |
| Iyonke | 50 μL |
Amanqaku:
1) a.I-10 × I-Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2) b.I-template ye-nucleic acid inconywa ukuba ichithwe kwi-DEPC yamanzi.
I-Hyasen Biotechnology Co., Ltd
3) Inkqubo yokusabela
| Ubushushu | Ixesha | Imijikelo |
| 65℃ | 60s a | 30 |
Amanqaku:
1. Qokelela uphawu lwe-fluorescent
Amanqaku
1. Ubushushu bokusabela bunokulungiswa phakathi kwe-62 ° C kunye ne-68 ° C ngokwe-primeriimeko;
2. Uvavanyo kufuneka lulungelelanise ukusebenza, kubandakanywa nokulungiswa kwenkqubo yokusabela,ukusetyenzwa kwesampula kunye nomthamo;
3. Ukuze ugweme ukungcola, kucetyiswa ukuba ulungiselele inkqubo yokusabela kwitafile ecocekileyo kakhulukwaye ungeze iitemplates kwi-fume hood kwamanye amagumbi ukunqanda ukuphazamiseka okungalunganga.
