I-Ribonuclease A (RNase A)

I-Ribonuclease A (i-RNase A) yi-polypeptide ene-stranded enye equkethe i-4 disulfide bond kunye ne-molecular weight malunga ne-13.7 kDa.I-RNase A yi-endoribonuclease eyehlisa ngokukodwa i-RNA enemisonto enye kwi-C kunye neentsalela ze-U.Ngokukodwa, i-cleavage iyaqaphela ibhondi ye-phosphodiester eyenziwe yi-5'-ribose ye-nucleotide kunye neqela le-phosphate kwi-3'-ribose ye-nucleotide ekufutshane ye-pyrimidine, ukwenzela ukuba i-2', 3' - i-cyclic phosphates i-hydrolyzed ehambelanayo. I-3'-nucleoside phosphates (umzekelo, i-pG-pG-pC-pA-pG icandwe yiRNase A ukuvelisa i-pG-pG-pCp kunye ne-A-PG).I-RNase A yeyona nto isebenzayo ekucaluleni i-RNA ene-stranded enye kwaye iyasebenza kwiimeko ezahlukeneyo zokusabela: kwi-concentration yetyuwa ephantsi (0 ukuya kwi-100 mM NaCl), ingasetyenziselwa ukucanda i-RNA enemisonto enye, i-RNA ephindwe kabini, kunye nemicu ye-RNA kwi-RNA-DNA hybrids.ngelixa kugxininiso lwetyuwa ephezulu (≥0.3 M), i-RNase A inokuqhawula ngokukodwa i-RNA ene-stranded single.