I-dATP/ dCTP/ dGTP/ dTTP/ dUTP (100mM )
Inkcazo
Le mveliso isisombululo solwelo esingenambala.Ilungele imifuniselo eyahlukeneyo eqhelekileyo yebhayoloji yemolekyuli efana nokwandiswa kwe-PCR, i-PCR yexesha lokwenyani, i-cDNA okanye i-DNA synthesis eqhelekileyo, ulandelelwano lwe-DNA kunye nokuleyibhelishwa.Iyakwazi ukuhlanjululwa ngamanzi ahlambulukileyo, kwaye ilungiswe kwi-pH 7.0 kunye nesisombululo se-NaOH esicocekileyo, ngococeko ≥ 99% (HPLC).Emva kokufunyanwa, ayinayo i-DNase, i-RNase kunye ne-phosphotase.Ingasetyenziswa ngokuthe ngqo kwiindlela ezahlukeneyo zebhayoloji eziqhelekileyo zemolekyuli njengePCR.
Ubume beMichiza
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Imbonakalo | Sula isisombululo esingenambala |
| Ukugxila | 100mM ±3% |
| Ubunyulu (HPLC) | ≥99% |
| PH(22-25°C) | 7.0±0.1 |
| Ulawulo lomgangathoI-1.Olu lungiselelo alunakho ukungcoliswa kwe-DNase kunye ne-RNase 2. Le nto ininzi ye-dNTP iye yavavanywa ukusebenza nge-Taq DNA polymerase kunye ne-Pfu DNA polymerase. Le ininzi ye-d NTP iye yavavanywa ukusebenza nge-Q-PCR | |
Usetyenziso
Uluhlu olude lwe-PCR (20 kb)
Ukwenziwa kwe-cDNA kunye ne-RT-PCR
I-PCR yexesha langempela
I-PCR eqhelekileyo
Ukunyaniseka okuphezulu kwe-PCR
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina kwi -25～-15℃
Umhla wokuphinda ucetywe:iminyaka eyi-2
Amanqaku
1) Inokuchithwa kwiqondo lokushisa.Emva kokutshatyalaliswa, kufuneka igcinwe kwibhokisi ye-ice okanye i-ice bath.Emva kokusetyenziswa, kufuneka igcinwe kwi -25～-15 ℃ kwangoko.
2) Ukhuseleko kunye nempilo yakho, nceda unxibe iidyasi zaselebhu kunye neeglavu ezilahlwayo ukuze usebenze.
