Inyathelo elinye RT-qPCR probe kit

I-One Step qRT-PCR Probe Kit yenzelwe ngokukodwa i-qPCR esebenzisa ngokuthe ngqo i-RNA (umz. i-virus RNA) njengethempleyithi.Ukusebenzisa i-gene specific primers (GSP), i-reverse transcription kunye ne-qPCR inokugqitywa kwityhubhu enye, ukunciphisa kakhulu iinkqubo zemibhobho kunye nomngcipheko wokungcola.Inokuthi ingasebenzi kwi-55 ℃ ngaphandle kokuchaphazela ukusebenza kakuhle kunye novakalelo lwe-qRT-PCR.Ukudibanisa ukusebenza okuphezulu kwe-HiScript III Reverse Transcriptase kunye ne-Hot-start Champagne Taq DNA Polymerase, ene-buffering system, uvakalelo lokubona lwe-One Step qRT-PCR Probe Kit inokufikelela kwi-0.1 pg ye-RNA iyonke okanye ngaphantsi kwe-10 iikopi zeetemplates ze-RNA. kwaye inozinzo oluphezulu lwe-thermal.Inyathelo elinye qRT-PCR Probe Kit ibonelelwe kwi Master Mix.I-5 × One Step Mix iqulethe i-buffer ephuculweyo kunye ne-dNTP/dUTP Mix, kwaye ifanelekile kwiinkqubo zokubona izinto ezikhethekileyo ezisekelwe kwi-fluorescence ebhalwe probes (umz. TaqMan).