Inyathelo elinye RT-qPCR SYBR Green Kit

I-Universal Blue qPCR Master Mix (i-Dye Based) sisisombululo sangaphambili se-2 × real-time quantitative quantitative PCR ephawulwa bubuntununtunu obuphezulu kunye nokuchaneka, inombala oluhlaza okwesibhakabhaka, kwaye inesiphumo sokulandela isampulu yokongeza.Elona candelo le-Taq DNA polymerase isebenzisa i-antibody eshushu isiqalo ukuthintela ngokufanelekileyo ukukhulisa okungangqalanga ngenxa yokufakwa kwe-primer ngexesha lolungiselelo lwesampulu.Kwangaxeshanye, ifomula yongeza izinto ezikhuthazayo zokuphucula ukusebenza kakuhle kokukhulisa ukusabela kwe-PCR kunye nokulinganisa ukunyuswa kofuzo kunye nemixholo eyahlukeneyo yeGC (30 ~ 70%), ukuze i-PCR yobungakanani ifumane ubudlelwane obuhle bomgca kubungakanani obubanzi. ummandla.Le mveliso iqulethe i-ROX Passive Reference Dye ekhethekileyo, esebenza kuzo zonke izixhobo ze-qPCR.Akuyomfuneko ukulungelelanisa ukuxinana kwe-ROX kwizixhobo ezahlukeneyo.Kuyimfuneko kuphela ukongeza iiprimers kunye neetemplates ukulungiselela inkqubo yokusabela yokukhulisa.