I-β–Nicotinamide Adenine Dinucleotide (NAD)
Iingenelo
1.Ukunyibilika kakuhle kwamanzi
2.Uzinzo oluhle.
Inkcazo
I-β-NAD + yi-coenzyme ye-dehydrogenase, kwaye i-β-NAD + ifumana i-hydrogen ngexesha lokuphendula kwaye iyanciphisa ngokwayo kwi-NADH.Njengesalathisi kunye ne-chromogen substrate, i-NADH ine-absorption peak kwi-340 nm, engasetyenziselwa ukufumanisa.
Ukulungiselela ii-reagents zophando lwezenzululwazi.Nge-NADH njengesalathisi kunye ne-chromogen substrate, kukho i-absorption peak kwi-340 nm, engasetyenziselwa ukufumanisa i-lactate dehydrogenase, i-transaminase kunye nokunye.
Ulwakhiwo lweMichiza
Ukubona ubude bobude
λ ubuninzi (unikezelo lombala)= 260 nm
Inkcazo
Izinto zovavanyo | Iinkcukacha |
Inkcazo | Umgubo omhlophe |
Uvavanyo (isiseko esomileyo) | ≥97% |
Ubunyulu(HPLC) | ≥99% |
Umxholo wesodium | ≤1% |
Isiqulatho samanzi | ≤5% |
PH ixabiso (100mg/ml yamanzi) | 2.0-4.0 |
IMethanol | ≤0.05% |
I-Ethanol | ≤1% |
Ubalo lwemicrobial lulonke | ≤750CFU/g |
Ukuthutha kunye nokugcinwa
Ezothutho:I-Ambient
UGcino kunye nozinzo:I-2-8 ° C, ivaliwe, yomile kwaye ikhuselwe ekukhanyeni.Ukugcina ixesha elide, kuyacetyiswa ukuba ugcinwe kwi -20 °C kwaye ukhuselwe ekukhanyeni.
Ubomi obucetyiswayo bokuvavanya kwakhona:Iminyaka emi-2