I-D-Lactate dehydrogenase (LDH)
Inkcazo
I-enzyme isetyenziselwa ukukhutshwa kwe-pyruvate ekunqumeni ukusebenza kunye ne-NADH (i-triglycerides, i-lipase, i-aldolase, i-aspartate aminotransferases, i-glutamate dehydrogenase).
Ukusetyenziswa
I-1.I-alkaline phosphatase edibene neeprotheni (i-antibodies, i-streptavidin njl,) inokuchonga ngokuthe ngqo iamolekyu ezijoliswe kuyo, kwaye ingasetyenziselwa kwi-ELISA, i-WB kunye nokufumanisa i-histochemical;
I-2.I-alkaline phosphatase ingasetyenziselwa ukukhupha i-5 '-terminal ye-DNA okanye i-RNA ukuthintela ukuzidibanisa;
3.I-DNA ye-dephosphorylated engentla okanye i-RNA ingabhalwa nge-radio-labeled phosphates (nge-T4 poly-nucleotide kinase)
Ulwakhiwo lweMichiza
Indlela yokusabela
D-Lactate + NAD+→ IPyruvate +NADH + H+
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Umgubo omhlophe we-amorphous, u-lyophilized |
| Umsebenzi | ≥200U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg umgubo/mL) | Cacileyo |
| I-NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| I-Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Ukuthutha kunye nokugcinwa
Ezothutho:AmbIent
Ugcino :Gcina kwi -20 ° C (Ixesha elide), 2-8 ° C (ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi:Iminyaka emi-2
