I-Hexokinase (HK)
Inkcazo
Sebenzisa i-Hexokinase ukuzimisela kwe-D-glucose, i-D-fructose, kunye ne-D-sorbitol ekutyeni okanye kwiisampuli zophando lwebhayoloji.I-enzyme ikwasetyenziselwa uvavanyo lwezinye i-saccharides eziguqulelwa kwi-glucose okanye i-fructose, kwaye ngoko ke luncedo ekuvavanyeni kwee-glycosides ezininzi.
Ukuba i-Hexokinase isetyenziswe ngokudibeneyo ne-glucose-6-phosphate dehydrogenase (G6P-DH) * (i-assays glucose6-phosphate eyenziwe yi-Hexokinase), iisampuli akufanele zibe yi-phosphate concentrations ephezulu njengoko i-G6P-DH ivinjelwa ngokukhuphisana ne-phosphate.
Ulwakhiwo lweMichiza
Umgaqo wokusabela
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Mhlophe ukuya kumthubi kancinane umgubo amorphous, lyophilized |
| Umsebenzi | ≥30U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg umgubo/ml) | Cacileyo |
| IiProteases | ≤0.01% |
| ATPase | ≤0.03% |
| I-Phosphoglucose isomerase | ≤0.001% |
| I-Creatine phosphokinase | ≤0.001% |
| IGlucose-6-Phosphate dehydrogenase | ≤0.01% |
| I-NADH/NADPH oxidase | ≤0.01% |
Ukuthutha kunye nokugcinwa
Ezothutho: Akufutshane
Ugcino :Gcina kwi -20 ° C (Ixesha elide), 2-8 ° C (ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi:1 unyaka
