I-Recombinant Deoxyribonuclease I (DNase I, RNase-free)
Inkcazo
I-Recombinant Deoxyribonuclease I, yi-endonuclease ekwazi ukwetyisa i-DNA enemisonto enye okanye ephindwe kabini.I-hydrolyzes i-phosphodiester bonds ukuvelisa i-mono- kunye ne-oligodeoxynucleotides equkethe amaqela angama-5'-phosphate kunye namaqela e-3'-OH.Uluhlu lwe-pH olusebenzayo lwe-DNase I luyi-7-8.Umsebenzi we-DNase I uxhomekeke kwi-Ca2 + kwaye inokuthi isebenze nge-ioni zetsimbi ze-divalent ezifana ne-Co2 +, i-Mn2 +, i-Zn2 +, njl. Phambi kwe-Mg2 +, i-DNase ndiyakwazi ukusika i-DNA ephindwe kabini nakweyiphi na indawo;ngelixa kubukho be-Mn2 +, i-DNase ndiyakwazi ukusika i-DNA ephindwe kabini kwindawo enye, yenze iziphelo ezimnyama okanye i-nuclei ye-1-2 i-Sticky iphetha ngee-nucleotide overhangs.Le enzyme ivela kwi-recombinant E. coli strains, ayiqulathanga RNase, kwaye ingasetyenziselwa ukusetyenzwa kweisampulu ezahlukeneyo zeRNA.
Ubume beMichiza
Usetyenziso
I-RNA extraction: lungiselela i-RNA ngaphandle kweDNA;
Emva kwe-in vitro transcription kunye ne-RNA polymerase, njenge-T7 RNA Polymerase (Cat # 10618), isetyenziselwa ukususa i-template ye-DNA;
Lungiselela i-DNA-free RNA phambi kwe-RT-PCR kunye ne-RT-qPCR;
Isetyenziswe ngokubambisana ne-DNA Polymerase I (Cat#12903) yokubhala iDNA ngokuguqulelwa kwe-nick;
Isetyenziswe kuhlalutyo lweenyawo ukuhlalutya ukusebenzisana kwe-DNA-protein;
Yenza ithala leencwadi eliqhekezayo;
Ekubhaqweni kwe-TUNEL ye-apoptosis, i-DNA ye-genomic echetyiweyo isetyenziswe njengolawulo olulungileyo.
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina kwi -20 ℃
Beka ubomi kwishelufa:iminyaka eyi-2
Amanqaku:
1.I-Enzymes kufuneka igcinwe kwibhokisi ye-ice okanye kwindawo yokuhlamba i-ice xa isetyenziswa, kwaye kufuneka igcinwe kwi -20 ° C ngokukhawuleza emva kokusetyenziswa.
2.Ukhuseleko kunye nempilo yakho, nceda unxibe izixhobo zokukhusela (i-PPE), njengeengubo zebhubhu kunye neeglavu ezilahlayo, xa usebenza nale mveliso.
