IHomocysteine(HCY)
Inkcazo
I-Homocysteine (HCY) isetyenziselwa ukufumanisa i-homocysteine egazini lomntu.I-Homocysteine (i-Hcy) i-amino acid enesulfure eveliswa yi-metabolism ye-methionine.I-80% ye-Hcy ibophelelwe kwiiprotheni ngokusebenzisa iibhondi ze-disulfide egazini, kwaye inxalenye encinci ye-homocysteine ekhululekile ithatha inxaxheba ekujikelezeni.Amanqanaba e-Hcy anxulumene ngokusondeleyo kwizifo zentliziyo.yinto ebalulekileyo yomngcipheko wesifo senhliziyo.Ukunyuka kwe-Hcy egazini kuvuselela udonga lwegazi ukuba lubangele umonakalo kwisitya se-arterial, okukhokelela ekudumbeni kunye nokwakhiwa kwe-plaque kudonga lwenqanawa, ekugqibeleni lukhokelela ekuvalelekeni kokuhamba kwegazi entliziyweni.Kwizigulane ezine-hyperhomocystinuria, iziphene ezinzima zofuzo zichaphazela i-Hcy metabolism, ekhokelela kwi-hyperhomocysteinemia.Iziphene zofuzo ezincinci okanye ukunqongophala kwezondlo zevithamini B ziya kuhamba kunye nokuphakama okuphakathi okanye okuncinci kwe-Hcy, okuya kwandisa ingozi yesifo senhliziyo.I-Hcy ephakamileyo inokubangela iziphene zokuzalwa ezifana ne-neural tube defects kunye nokukhubazeka kokuzalwa.
Ulwakhiwo lweMichiza
Umgaqo wovavanyo
I-Hcy ene-Oxidized iguqulwa ibe yi-Hcy yamahhala, kwaye i-Hcy yamahhala isabela nge-serine phantsi kwe-catalysis ye-CBS ukuvelisa i-L-cystathionine.I-L-cystathionine ivelisa i-Hcy, i-pyruvate kunye ne-NH3 phantsi kwe-catalysis ye-CBL.I-pyruvate eyenziwa yile mpendulo yomjikelezo ingabonwa nge-lactate dehydrogenase LDH kunye ne-NADH, kwaye izinga lokuguqulwa kwe-NADH kwi-NAD lihambelana ngokuthe ngqo nomxholo we-Hcy kwisampuli.
Ukuthutha kunye nokugcinwa
Ezothutho:2-8°C
Ugcino kunye nexesha lokuqinisekisa:Ama-reagents angavulwanga kufuneka agcinwe kwi-2-8 ° C ebumnyameni, kwaye ixesha lokuqinisekisa ziinyanga ezili-12;emva kokuvula, i-reagents kufuneka igcinwe ebumnyameni kwi-2-8 ° C, kwaye ixesha lokuqinisekisa liyinyanga eyi-1 phantsi kwemeko yokungabikho kokungcola;i-reagents akufanele ifakwe emkhenkceni.
Phawula
Iimfuno zesampulu: Isampuli yi-serum entsha okanye i-plasma (i-heparin anticoagulation, i-0.1mg ye-heparin inokuthintela igazi le-1.0ml).Nceda i-centrifuge i-plasma ngokukhawuleza emva kokuqokelela igazi, okanye ifriji kunye ne-centrifuge ngaphakathi kweyure ye-1.
