IGlycerol Kinase(GK)
Inkcazo
Iprotheyini efakwe kule geni yeyosapho lweFGGY kinase.Le protheni iyi-enzyme ephambili ekulawuleni ukufunyanwa kwe-glycerol kunye ne-metabolism.Inceda i-phosphorylation ye-glycerol nge-ATP, ivelise i-ADP kunye ne-glycerol-3-phosphate.Ukuguquka kwesi mfuza kuhambelana nokusilela kwe-glycerol kinase (GKD).Kungenjalo, ukwahluka okukhutshelweyo okudityanisiweyo okufakelweyo kwii-isoform ezahlukeneyo kufunyenwe kulo mzobo.
Le enzyme isetyenziselwa iimvavanyo zokuxilonga ukumiselwa kwe-triglycerides kunye ne-Glycerol-3-phosphate Oxidase.
Ulwakhiwo lweMichiza
Umgaqo wokusabela
Glycerol + ATP→ Glycerol -3-phosphate + ADP
Inkcazo
Izinto zovavanyo | Iinkcukacha |
Inkcazo | White ukuya kumgubo kancinane amorphous tyheli, lyophilized |
Umsebenzi | ≥15U/mg |
Ubunyulu(SDS-PAGE) | ≥90% |
Ukunyibilika (10mg umgubo/ml) | Cacileyo |
IsiCatalase | ≤0.001% |
IGlucose oxidase | ≤0.01% |
Uricase | ≤0.01% |
ATPase | ≤0.005% |
Hexokinase | ≤0.01% |
Ukuthutha kunye nokugcinwa
Ezothutho:Ithunyelwe ngaphantsi kwe-15°C
Ugcino :Gcina kwi -20°C(Ixesha elide), 2-8°C(Ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi:Iinyanga ezili-18