I-Phosphatase Alkaline (ALP)
Inkcazo
I-Alkaline Phosphatase ithathwa kwi-recombinant E. coli strain ethwala i-TAB5 gene.I-enzyme ivuselela i-dephosphorylation ye-5' kunye ne-3' ekupheleni kwe-DNA kunye ne-RNA phosphomonoesters.Kwakhona, i-hydrolyses ribose, kunye ne-deoxyribonucleoside triphosphates (NTPs kunye ne-dNTPs).I-TAB5 i-Alkaline Phosphatase isebenza ku-5' ukuphumela ngaphandle, 5' ukuhla kunye neziphelo ezibuthuntu.I-Phosphatase ingasetyenziswa kwizicelo ezininzi zebhayoloji yemolekyuli, ezinje nge-cloning okanye ukuphonononga iilebhile zokuphelisa ukususa iziphelo ze-phosphorylated ze-DNA okanye i-RNA.Kwiimvavanyo ze-cloning, i-dephosphorylation ikhusela i-DNA ye-plasmid yomgca kwi-self-ligation.Isenokwehlisa umgangatho we-dNTPs ezingadityaniswanga kwiimpendulo ze-PCR ukulungiselela itemplate yokulandelelana kwe-DNA.I-enzyme yenziwe ngokupheleleyo kwaye ngokungenakuguquleka isebenze ngokufudumeza kwi-70 ° C imizuzu emi-5, ngaloo ndlela isusa i-phosphatase ngaphambi kokubopha okanye ukuphelisa ukuleyibhile ngokungeyomfuneko.
Ukusetyenziswa
I-1.I-alkaline phosphatase edibene neeprotheni (i-antibodies, i-streptavidin njl,) inokuchonga ngokuthe ngqo iamolekyu ezijoliswe kuyo, kwaye ingasetyenziselwa kwi-ELISA, i-WB kunye nokufumanisa i-histochemical;
I-2.I-alkaline phosphatase ingasetyenziselwa ukukhupha i-5 '-terminal ye-DNA okanye i-RNA ukuthintela ukuzidibanisa;
3.I-DNA ye-dephosphorylated engentla okanye i-RNA ingabhalwa nge-radio-labeled phosphates (nge-T4 poly-nucleotide kinase)
Ulwakhiwo lweMichiza
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Umsebenzi we-Enzyme | 5U/μL |
| Umsebenzi we-Endonuclease | Ayifunyaniswanga |
| Umsebenzi we-Exonuclease | Ayifunyaniswanga |
| Umsebenzi wokudlala | Ayifunyaniswanga |
| Umsebenzi we-RNase | Ayifunyaniswanga |
| E.coli DNA | ≤1ikopi/5U |
| Endotoxin | Uvavanyo lwe-LAL, ≤10EU/mg |
| Ubunyulu | ≥95% |
Ukuthutha kunye nokugcinwa
Ezothutho:AmbIent
Ugcino :Gcina kwi-2-8°C
Uvavanyo kwakhona olucetyiswayoUbomi:Iminyaka emi-2
