I-ELEISA KIT ye-Trypsin
Inkcazo
I-Recombinant trypsin isetyenziswa rhoqo kwimveliso ye-biopharmaceutical-ngexesha lokulungiswa kweeseli okanye ukuguqulwa kunye nokusebenza kweemveliso.I-Trypsin ibeka imingcipheko yokhuseleko kwaye ke kufuneka isuswe ngaphambi kokukhutshwa kwemveliso yokugqibela.Le Kithi yeSandwich yeyomlinganiselo wobungakanani be-Trypsin eNshiyekileyo kwi-cell culture supernatant kunye nezinye iinkqubo zokwenziwa kwe-biopharmaceutical xa kusetyenziswa i-Trypsin.
Le khithi yi-Enzyme-Linked Immunosorbent Assay (ELISA).Ipleyiti iqatywe ngaphambili ngePorcine trypsin antibody.I-Trypsin ekhoyo kwisampulu yongezwa kwaye ibophelela kwii-antibodies ezigqunywe emaquleni.Kwaye emva koko i-biotinylated ye-Porcine trypsin antibody yongezwa kwaye ibophelela kwi-trypsin kwisampulu.Emva kokuhlamba, i-HRP-Streptavidin yongezwa kwaye ibophelela kwi-antibody ye-Biotinylated trypsin.Emva kofukamo olungabotshwanga i-HRP-Streptavidin iyahlanjwa.Emva koko isisombululo se-substrate ye-TMB yongezwa kwaye ifakwe yi-HRP ukuvelisa imveliso yombala ozuba oye watshintsha waba tyheli emva kokongeza isisombululo se-acidic stop.Ubuninzi betyheli bulinganiswe kwisixa ekujoliswe kuso se-trypsin
isampuli ifakwe kwipleyiti.I-absorbence ilinganiswa kwi-450 nm.
Ulwakhiwo lweMichiza
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Imbonakalo | Gqibezela ukupakisha kwaye akukho kuvuza kwamanzi |
| Umda osezantsi wokufunyanwa | 0.003 ng/mL |
| Umda osezantsi wobungakanani | 0.039 ng/mL |
| Ukuchaneka | I-Intra assay CV≤10% |
Ukuthutha kunye nokugcinwa
Ezothutho:I-Ambient
Ugcino:Ingagcinwa kwi -25 ~ -15 °C kubomi beshelufa, 2-8 °C ukwenzela olunye uvavanyo olulula
Ubomi obucetyiswayo bokuvavanya kwakhona:1 unyaka
