I-Deoxyribonuclease I(Dnase I)

I-DNase I (i-Deoxyribonuclease I) yi-endodeoxyribonuclease ekwazi ukwetyisa i-DNA enemisonto emibini.Iqaphela kwaye iqhawule iibhondi ze-phosphodiester ukuvelisa i-monodeoxynucleotides okanye i-oligodeoxynucleotides enye okanye kabini kunye namaqela e-phosphate kwi-5'-terminal kunye ne-hydroxyl kwi-3'-terminal.Umsebenzi we-DNase I ixhomekeke kwi-Ca 2 + kwaye inokuthi isebenze nge-ion zetsimbi ze-divalent ezifana ne-Mn 2 + kunye ne-Zn 2 +.I-5 mM Ca 2+ ikhusela i-enzyme kwi-hydrolysis.Xa kukho iMg 2+, i-enzyme inokubona ngokungenamkhethe kwaye icande nayiphi na indawo kuwo nawuphi na umcu weDNA.Phambi kobukho be-Mn 2+, imicu ephindwe kabini ye-DNA inokuqatshelwa ngaxeshanye kwaye icandeke phantse kwindawo enye ukuze yenze i-flat end DNA fragments okanye isiphelo esincangathi se-DNA fragments kunye ne-1-2 nucleotides ephumayo.