I-Cholesterol oxidase(COD/CHOD)
Inkcazo
I-Cholesterol oxidase (CHOD) yenza inyathelo lokuqala kwi-cholesterol catabolism.Ezinye iibhaktheriya ezingekho kwi-pathogenic, ezifana ne-Streptomyces ziyakwazi ukusebenzisa i-cholesterol njengomthombo wekhabhoni.Iibhaktheriya ze-Pathogenic, ezifana ne-Rhodococcus equi, zifuna i-CHOD ukuba ihlasele i-macrophage yomninimzi.I-CHOD iyasebenza kabini.I-cholest-5-en-3-enye i-isomerized ukuya kwi-cholest-4-en3-one.I-reaction ye-isomerization inokuguqulwa ngokuyinxenye.Umsebenzi we-CHOD uxhomekeke kwiipropati ezibonakalayo ze-membrane apho i-substrate iboshiwe.
I-CHOD isetyenziselwa ukumisela i-cholesterol ye-serum.Yesibini i-enzyme esetyenziswa kakhulu kwizicelo zokuxilonga emva kwe-glucose oxidase.I-CHOD iphinda ifumane isicelo kwi-microanalysis ye-steroids kwiisampuli zokutya kunye nokuhlukanisa i-3-ketosteroids ukusuka kwi-3b-hydroxysteroids.Izityalo zeTransgenic ezibonisa i-cholesterol oxidase ziphandwa ekulweni ne-cotton boll weevil.I-Cholesterol oxidase nayo isetyenziswe njengeprobe ye-molecular ukucacisa izakhiwo ze-cellular membrane.
Ulwakhiwo lweMichiza
Umgaqo wokusabela
ICholesterol + O2 →△4-Cholesten-3-enye + H2O2
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Yellowish amorphous powder, lyophilized |
| Umsebenzi | ≥8U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg umgubo/ml) | Cacileyo |
| IsiCatalase | ≤0.001% |
| IGlucose oxidase | ≤0.01% |
| I-Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Ukuthutha kunye nokugcinwa
Ezothutho:Ithunyelwe ngenqanawa ngaphantsi kwe -15°C
Ugcino :Gcina kwi -25~-15°C(Ixesha elide), 2-8°C(Ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi:1 unyaka
