Bst 2.0 DNA Polymerase (iGlycerol isimahla, inxinano ephezulu)
I-Bst DNA polymerase V2 ivela kwi-Bacillus stearothermophilus DNA Polymerase I, enomsebenzi we-5′→3′ DNA polymerase kunye nomsebenzi oqinileyo wokutshintshwa kwekhonkco, kodwa akukho 5′→3′ umsebenzi we-exonuclease.I-Bst DNA Polymerase V2 ifanelekile ngokufanelekileyo kwi-strand-displacement, isothermal amplification LAMP (i-Loop mediated isothermal amplification) kunye nokulandelelana ngokukhawuleza.Le Bst DNA polymerase V2 iyakwazi ukuthintela umsebenzi we-DNA polymerase kwindawo yokushisa, ukuze iqhutywe kwaye inkqubo yokuphendula ingenziwa kwiqondo lokushisa, ukuthintela ukukhulisa okungaqhelekanga kunye nokuphucula ukusebenza kakuhle kokuphendula, kwaye le nguqulo ingakwazi ube lyophilized.Ukongeza, iyakwazi ukukhulula umsebenzi wayo kumaqondo okushisa aphakamileyo, ngaloo ndlela isusa imfuno yesinyathelo sokuvula esahlukileyo.
Amacandelo
| Icandelo | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| I-Bst DNA polymerase V2 (iGlycerol-free) (32U/μL) | 0.05 mL | 0.25 ml | 2.5 ml |
| I-10 × HC Bst V2 Buffer | 1.5 ml | 2×1.5 mL | 3×10 mL |
| MgSO4 (100mM) | 1.5 ml | 2×1.5 mL | 2×10 mL |
Usetyenziso
1.I-LAMP isothermal amplification
2.I-DNA strand single displacement reaction
3.Ulandelelwano lwemfuza yeGC ephezulu
4.Ukulandelelana kwe-DNA kwinqanaba le-nanogram.
Imeko yoGcino
Ukuthutha ngaphantsi kwe-0°C kwaye igcinwe kwi-25°C~-15°C.
Inkcazelo yeyunithi
Iyunithi enye ichazwa njengobungakanani be-enzyme edibanisa i-25 nmol ye-dNTP kwizinto ezingenayo i-asidi kwimizuzu engama-30 kwi-65 ° C.
Isibane Reaction
| Amacandelo | 25 μLInkqubo |
| I-10 × HC Bst V2 Buffer | 2.5 μL |
| MgSO4 (100mM) | 1.5 μL |
| dNTPs (10mM nganye) | 3.5 μL |
| I-SYTO™ 16 eluhlaza (25×)a | 1.0 μL |
| I-Primer mixb | 6 μL |
| I-Bst DNA Polymerase V2 (iGlycerol-free) (32 U/uL) | 0.25 μL |
| Isifanekiso | × μL |
| ddH₂O | Ukuya kuthi ga kwi-25 μL |
Amanqaku:
1) a.I-SYTO TM 16 eluhlaza (25 ×): Ngokweemfuno zovavanyo, ezinye iidayi zingasetyenziswa njengeendawo;
2) b.I-Primer mix: ifunyenwe ngokuxuba i-20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB kunye neminye imiqulu.
Ukusabela kunye neMeko
1 × HC Bst V2 Buffer, ubushushu bokufukamela buphakathi kwama-60°C kunye nama-65°C.
Ukushisa Ukungasebenzi
80°C, imizuzu engama-20.
