Virus DNA/RNA Extraction Kit
Ikhithi (HC1009B) inokukhawuleza ikhuphe i-high-purity viral nucleic acids (DNA/RNA) kwiisampulu ezahlukeneyo zolwelo ezifana negazi, i-serum, iplasma, kunye ne-swab yokuhlamba ulwelo, ivumela ukusetyenzwa okuphezulu kweesampulu ezihambelanayo.Ikhithi isebenzisa amaso amagnetic e-superparamagnetic esekwe kwisilicon.Kwinkqubo ye-buffer ekhethekileyo, ii-nucleic acids endaweni yeeprotheyini kunye nobunye ukungcola zibhengezwa ngeebhondi ze-hydrogen kunye nokubopha i-electrostatic.Amaso amagnetic ane-adsorbed nucleic acids ahlanjwa ukuze asuse iiprotheyini eziseleyo kunye neetyuwa.Xa usebenzisa i-buffer yetyuwa ephantsi, i-nucleic acids ikhutshwa kwi-beads magnetic, ukuze kufezekiswe injongo yokwahlula ngokukhawuleza kunye nokuhlanjululwa kwe-nucleic acids.Yonke inkqubo yokusebenza ilula, ikhawuleza, ikhuselekile kwaye iyasebenza, kwaye i-nucleic acids efunyenweyo ingasetyenziselwa ngokuthe ngqo kwiimvavanyo ezisezantsi ezifana ne-reverse transcription, i-PCR, i-qPCR, i-RT-PCR, i-RT-qPCR, ukulandelelana kwesizukulwana esilandelayo, uhlalutyo lwe-biochip, njl.
Iimeko zokugcina
Gcina kwi-15 ~ 25 ℃, kunye nokuthutha kwindawo yokushisa.
Usetyenziso
Igazi, i-serum, iplasma, i-swab eluent, i-tissue homogenate kunye nokunye.
Inkqubo yoVavanyo
1. Isampuli ukuqhubekeka
1.1 Kwiintsholongwane kwiisampulu zolwelo ezifana negazi, i-serum, kunye neplasma: 300μL ye-supernatant esetyenziselwa ukutsalwa.
2.2 Kwiisampulu zeswab: Beka iisampulu zeswab kwiityhubhu zesampulu eziqulethe isisombululo sokugcina, i-vortex ye-1 min, kwaye uthathe i-300μL ye-supernatant ukuze itsalwe.
1.3 Kwiintsholongwane kwi-tissue homogenates, izisombululo ze-tishu, kunye neesampuli zokusingqongileyo: Yima iisampulu ze-5 -10 min, kwaye uthathe i-300μL ye-supernatant ukuze ikhutshwe.
2. Ukulungiswa kwe ukulungiselelai-reagent eqinisekisiwe
Khupha iirejenti ezipakishwe ngaphambili kwikhithi, guqula kwaye udibanise amaxesha amaninzi ukumisa kwakhona amaso kazibuthe.Vuthulula ngobunono ipleyiti ukwenza iirejenti kunye namaso azibuthe zitshone ezantsi equleni.Nceda uqinisekise icala lepleyiti kwaye ukrazule ngononophelo ifoyile yealuminiyam yokutywina.
Δ Kuphephe ukungcangcazela xa ukrazula ifilim yokutywina ukunqanda ukuchitheka kolwelo.
3. Ukusebenza kwe i-automisixhobo se-atic
3.1 Yongeza i-300μL yesampuli kumaqula kwiiKholam 1 okanye 7 ze-96 ipleyiti enzulu yequla (nikela ingqalelo kwindawo esebenzayo yokusebenza kakuhle).Umthamo wegalelo lesampuli iyahambelana ne-100-400 μL.
3.2 Beka ipleyiti enzulu yequla engama-96 kwi-nucleic acids extractor.Beka imikhono yebha yemagnethi, kwaye uqinisekise ukuba igquma ngokupheleleyo iintonga zemagnethi.
3.3 Misela inkqubo ngolu hlobo lulandelayo ukulungiselela utsalo oluzenzekelayo:
3.4 Emva kokutsalwa, tshintshela i-eluent ukusuka kuKholam 6 okanye 12 we-96 deep well plate (nikela ingqalelo kwindawo esebenza kakuhle yokusebenza kakuhle) ukuya kwityhubhu ecocekileyo ye-Nuclease-free centrifuge.Ukuba awuyisebenzisi ngokukhawuleza, nceda ugcine iimveliso ku -20 ℃.
Amanqaku
Isetyenziselwa uphando kuphela.Ayisetyenziswa kwiinkqubo zokuxilonga.
1. Imveliso ekhutshiweyo yi-DNA / RNA.Ingqwalasela ekhethekileyo kufuneka ihlawulwe ukukhusela ukuthotywa kwe-RNA nge-RNase ngexesha lokusebenza.Izitya kunye neesampulu ezisetyenzisiweyo kufuneka zinikezelwe.Zonke iityhubhu kunye neengcebiso ze-pipette kufuneka zifakwe inzalo kunye ne-DNase / RNase-free.Abanini-zithuthi kufuneka banxibe iiglavu ezingenamgubo kunye nemaski.
2. Kucelwa ufunde incwadana yemiyalelo ngocoselelo phambi kokuba uyisebenzise, kwaye usebenze ngokungqinelana ngqo nencwadana yemiyalelo.Ukusetyenzwa kwesampuli kufuneka kuqhutywe kwibhentshi ecocekileyo kakhulu okanye kwikhabhinethi yokhuseleko lwebhayoloji.
3. Inkqubo yokukhutshwa kwe-nucleic acid ngokuzenzekelayo kufuneka ihlanjululwe yi-UV ye-30 min ngaphambi nangemva kokusetyenziswa.
4. Kusenokubakho umkhondo wamaso kazibuthe oseleyo kwi-eluent emva kokutsalwa, ngoko ke kuphephe ukunqwenela amaso kazibuthe.Ukuba amaso amagnetic anqweneleka, anokususwa ngokuma kwemagnethi.
5. Ukuba akukho miyalelo ikhethekileyo yeebhetshi ezahlukeneyo zeerejenti, nceda ungazixubi, kwaye uqinisekise ukuba iikhithi zisetyenziswa ngaphakathi kwexesha elisemthethweni.
6. Lahla ngokufanelekileyo zonke iisampuli kunye ne-reagent, sula ngokucokisekileyo kwaye ubulale iintsholongwane kuzo zonke iindawo zokusebenza nge-75% ye-ethanol.
