Taq DNA Anti-Body
I-Taq DNA Antibody sisithintelo esiphindwe kabini se-Taq DNA Polymerase monoclonal antibody ye-PCR yokuqala eshushu.Inokuthintela umsebenzi we-5′→3′ polymerase kunye ne-5′→3′ exonuclease emva kokubophelela kwi-Taq DNA Polymerase, enokuthi ithintele ngokufanelekileyo i-annealing engabonakaliyo ye-primers kunye nokukhulisa okungacaciswanga okubangelwa yi-primer dimer kwiqondo lokushisa eliphantsi.Ukongeza, imveliso inokuthintela ngokufanelekileyo ukuthotywa kweprobe.I-Taq DNA Antibody yenziwa idenaturation kwinyathelo lokuqala le-DNA denaturation ye-PCR reaction, apho umsebenzi we-DNA polymerase ubuyiselwa ukuphumeza umphumo we-PCR yokuqala eshushu.Ingasetyenziswa phantsi kwemeko yokusabela kwe-PCR yesiqhelo ngaphandle kokwenziwa kusebenze okukhethekileyo kwe-antibody.
Imeko yoGcino
Imveliso ithunyelwa ngeepakethe ze-ice kwaye ingagcinwa kwi -25 ° C ~ -15 ° C iminyaka eyi-2.
Usetyenziso
Ukugxininiswa kwale mveliso ngu-5 mg / mL.I-1 μL i-antibody inokuthintela umsebenzi we-20-50 U Taq DNA polymerase.Kucetyiswa ukuxuba i-antibody kunye ne-Taq DNA polymerase kwiqondo lobushushu begumbi iyure enye (ifukame kwiqondo lobushushu legumbi iiyure ezi-2 xa umthamo ungaphezulu kwe-200 mL, kwaye umthengi kufuneka ahlengahlengise inkqubo xa efakwe kumthamo omkhulu), kwaye emva koko agcine. ku -20 ℃ ngobusuku phambi kokusetyenziswa.
Qaphela: Umsebenzi othile we-Taq DNA Polymerase eyahlukileyo iyahluka, umlinganiselo wokuthintela kufuneka ulungelelaniswe ngokufanelekileyo ukuze ufezekise ukuba ukusebenza kakuhle kokuthintela kungcono kune-95%.
Iinkcukacha
| Ukuhlelwa | I-Monoclonal |
| Uhlobo | I-antibody |
| I-Antigen | Taq DNA Polymerase |
| Ifom | Ulwelo |
Amanqaku
Nceda unxibe iPPE eyimfuneko, idyasi yelebhu kunye neeglavu, ukuqinisekisa impilo nokhuseleko lwakho!
