I-RT-LAMP Fluorescent Master Mix (Amaso eLyophilized)
Ingcaciso yeMveliso
I-LAMP okwangoku isetyenziswa ngokubanzi iteknoloji kwintsimi ye-isothermal amplification.Isebenzisa ii-primers ze-4-6 ezinokuchonga imimandla ethile ye-6 kwi-gene ekujoliswe kuyo, kwaye ixhomekeke kumsebenzi oqinileyo wokufuduka kwe-strand ye-Bst DNA polymerase.Kukho iindlela ezininzi zokubona i-LAMP, kubandakanywa indlela yedayi, indlela ye-pH colorimetric, indlela ye-turbidity, i-HNB, i-calcein, njl njl. I-RT-LAMP yolunye uhlobo lwe-LAMP yokuphendula kunye ne-RNA njenge template.I-RT-LAMP Fluorescent Master Mix (i-Lyophilized Powder) ikwimo ye-Lyophilized Powder, kwaye idinga kuphela ukongeza iiprimers kunye neetemplates xa usebenzisa.
Inkcazo
Izinto zovavanyo | Iinkcukacha |
Endonulease | Akukho icinyiweyo |
Umsebenzi we-RNase | Akukho nanye ifunyenweyo |
Umsebenzi we-DNase | Akukho nanye ifunyenweyo |
Umsebenzi we-Nickase | Akukho nanye ifunyenweyo |
E. coli.gDNA | ≤10iikopi/500U |
Amacandelo
Le mveliso iqulethe i-Reaction Buffer, i-RT-Enzymes Mix ye-Bst DNA Polymerase kunye ne-Thermostable Reverse Transcriptase, i-Lyoprotectant kunye ne-Fluorescent Dye Components.
Ukulinganisa
I-Isothermal amplification ye-DNA kunye ne-RNA.
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:I-Ambient
Imiqathango yoGcino:Gcina kwi -20 ℃
Umhla wokuphinda ucetywe:Iinyanga ezili-18