I-RT-LAMP Colormetric (ibhola e-Lyophilized)
Le mveliso iqulethe reaction buffer, RT-Enzymes Mix (Bst DNA polymerase kunye nobushushu-resistant reverse transcriptase), izikhuseli lyophilized kunye amacandelo chromogenic idayi.Imveliso yibhola yohlobo lwe-lyophilized, usebenzisa kuphela iiprimers kunye neetemplates.Le khithi ibonelela ngokukhawuleza, ukukhangela okubonakalayo okucacileyo kokukhulisa, ukusabela okungalunganga kubonakaliswe kubomvu kunye nokusabela okulungileyo kuboniswa ngotshintsho oluphuzi.
Icandelo
I-RT-LAMP Colormetric Master Mix (amaso e-Lyophilized)
Usetyenziso
YeDNA okanye iRNA isothermal amplification.
Iimeko zokuGcina
Ithuthwe kwaye igcinwe kwi-2 ~ 8℃.Imveliso isebenza kwiinyanga ezili-12.
Umgaqo-nkqubo
1.Thatha inani elihambelanayo le-Lyophilized beads powder ngokwenani leemvavanyo.
2.Lungisa umxube wokusabela
| Icandelo | Umthamo |
| I-RT-LAMP Colormetric Master Mix (amaso e-Lyophilized) | Iqhekeza eli-1 (amaso ama-2) |
| 10 × I-Primer Mixa | 5 μL |
| Izakhelo zeDNA/RNA b | 45 μL |
Amanqaku:
1. 10 × I-Primer Mix Concentration: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2. Iitemplates ze-Nucleic acid zinconywa ukuba zichithwe ngokusebenzisa amanzi e-DEPC.
3.Fukamisa kwi-65 ° C kwi-30-45mins, enokwandiswa ngokufanelekileyo ngokutshintsha umbala Ixesha lokuphendula.
4.Ngokweliso lenyama, umthubi u-positive kwaye ubomvu bu-negative.
Amanqaku
1.Ubushushu bokusabela bunokulungiswa phakathi kwe-62 ℃ kunye ne-68 ℃ ngokwemeko ye-primer.
2.Ii-reagents ezipakishweyo akufanele zibekwe emoyeni ixesha elide.
3.Ukusabela kokutshintsha kombala obomvu notyheli kuxhomekeke kutshintsho lwe-pH yenkqubo yokusabela, nceda ungasebenzisi isisombululo sokugcina se-Tris nucleic acid, ekucetyiswa ukuba usebenzise i-ddH.2O i-nucleic acid egciniweyo.
4.Uvavanyo luya kuqhutywa ngendlela esemgangathweni, kubandakanywa ukulungiswa kwenkqubo yokusabela, unyango lwesampuli kunye nokudibanisa isampuli.
5.Kucetyiswa ukuba kulungiswe inkqubo yokusabela kwitafile ecocekileyo kakhulu kwaye wongeze itemplates kwifusi yomlilo yamanye amagumbi ukunqanda ubuxoki.
