I-Peroxidase (umthombo weHorseradish) Isithethantonye: I-Hydrogen peroxide oxidoreductase;HRP

I-Horseradish peroxidase (HRP) ihlukaniswe kwiingcambu ze-horseradish (Amoracia rusticana) kwaye iyingxenye ye-ferroprotoporphyrin ye-peroxidases.I-HRP idibanisa ngokukhawuleza kunye ne-hydrogen peroxide (H2O2).Isiphumo [i-HRP-H2O2] esintsonkothileyo sinokukhupha iindidi ezininzi zabanikeli be-hydrogen:
UMnikeli + H2O2 → uMnikeli oXhoxisiweyo + 2 H2O
I-HRP iya ku-oxidize substrates ezahlukeneyo (jonga iTheyibhile 1):
• Chromogenic
• I-Chemiluminescent (efana ne-luminol okanye i-isoluminol)
• I-Fluorogenic (efana ne-tyramine, i-homovanillic acid, okanye i-4-hydroxyphenyl acetic acid)
I-HRP yi-polypeptide yekhonkco enye equlethe iibhulorho ezine ze-disulfide.I-HRP yi-glycoprotein equlethe i-18% ye-carbohydrate.Ukwakheka kwecarbohydrate kubandakanya igalactose, arabinose, xylose, fucose, mannose, mannosamine, kunye negalactosamine, kuxhomekeke kwi-isozyme ethile.

I-HRP yileyibhile esetyenziswa ngokubanzi kwi-immunoglobulins kwizicelo ezininzi ezahlukeneyo ze-immunochemistry, kubandakanywa i-immunoblotting, i-immunohistochemistry, kunye ne-ELISA.I-HRP inokuhlanganiswa kwii-antibodies ngeendlela ezininzi ezahlukeneyo, kubandakanywa i-glutaraldehyde, i-oxidation ye-periodation, ngeebhondi ze-disulfide, kunye ne-amino kunye ne-thiol eqondiswe kwi-cross-linkers.I-HRP yeyona ileyibhile ifunwayo kwizilwa-buhlungu, kuba iyeyona incinci kwaye izinzileyo kwezona layibhile zintathu ze-enzyme (peroxidase, β-galactosidase, alkaline phosphatase) kwaye i-glycosylation yayo ikhokelela ekubopheni okungeyonto ethile.Uphononongo lwe-glutaraldehyde kunye neendlela zokudibanisa zexesha ziye zapapashwa.

I-Peroxidase ikwasetyenziselwa ukumisela i-glucose4 kunye neeperoxides kwisisombululo.Upapasho oluninzi, i-6-24 thesis, 25-29 kunye ne-30-46 dissertations bakhankanye ukusetyenziswa kwe-P8375 kwiiprothokholi zabo zophando.