Inyathelo elinye RT-qPCR probe kit
Inkcazo
U+ Inyathelo elinye RT-qPCR Probe Kit (Glycerol-free) is a glycerol-free inyathelo elinye RT-qPCR reagent usebenzisa RNA njenge template (ezifana RNA virus), leyo ilungele uphuhliso kunye noyilo iimveliso lyophilized.Le mveliso idibanisa ukusebenza okuphezulu kwenyathelo elinye elinikezelwe i-Reverse Transcriptase kunye ne-hot-start Champagne Taq DNA Polymerase, ene-chemical buffer eyomisa kakuhle, enomgangatho ongcono wokukhulisa, ibhalansi kunye neenkcukacha, kwaye iyahambelana nokumisa okwahlukileyo iinkqubo.Ukongezelela, inkqubo ye-dUTP / UDG yokuchasana nokungcola ifakwe kwi-reagent, enokusebenza kwiqondo lokushisa lokushisa, isuse impembelelo yokungcoliswa kwemveliso yokukhulisa kwi-qPCR, kwaye iqinisekise ukuchaneka kweziphumo.
Imigaqo esisiseko ye-RT-qPCR
Inkcazo
| Izinto zovavanyo | Inkcazo | Isiphumo |
| (SDS IPHEPHA) Ukucoceka kwesitokhwe se-Enzyme(SDS PAGE) | ≥95% | Dlula |
| Umsebenzi we-Endonuclease | Ayifunyaniswanga | Dlula |
| Umsebenzi we-Exodulease | Ayifunyaniswanga | Dlula |
| Umsebenzi weRnase | Ayifunyaniswanga | Dlula |
| Intsalela ye-E.coli DNA | ＜1 iikopi/60 | Dlula |
| Inkqubo yoVavanyo oluSebenzayo | 90%≤110% | Dlula |
Amacandelo
| Amacandelo | 100rxns | I-1,000rns | 5,000 rxns |
| RNase-free ddH2O | 2*1ml | 20ml | 100ml |
| 5*umxube wenyathelo elinye | 600μl | 6*1ml | 30ml |
| Inyathelo elinye lomxube we-enzyme | 150μl | 2*750μl | 7.5ml |
| 50* ROX reference Daye 1 | 60μl | 600μl | 3*1ml |
| 50* ROX isalathiso Idayi 2 | 60μl | 600μl | 3*1ml |
a.Isithinteli esineNqana-linye sibandakanya i-dNTP Mix kunye ne-Mg2+.
b.I-Enzyme Mix iqulethe ikakhulu umva
i-transcriptase, i-Hot Start Taq DNA polymerase (ukuguqulwa kwe-antibody) kunye ne-RNase inhibitor.
c.Isetyenziselwa ukulungisa impazamo yeesinal zefluorescene phakathi kwamaqula ahlukeneyo.
c.I-ROX: Kuya kufuneka ukhethe ulungelelwaniso ngokwemodeli yesixhobo sovavanyo.
Usetyenziso
Ukufunyanwa kwe-QPCR
Ukuthumela ngenqanawa kunye noGcino
Ezothutho:Iipaki zomkhenkce
Imiqathango yoGcino:Gcina kwi -20 ℃.
Ubomi beShief:Iinyanga ezili-18
