IGlucose-6-phosphate dehydrogenase (G6PDH)
Inkcazo
Ukunqongophala kweGlucose 6 phosphate dehydrogenase (G6PD) yimeko yofuzo apho iiseli ezibomvu zegazi ziqhekeka (i-hemolysis) xa umzimba ubonakaliswa kukutya okuthile, iziyobisi, usulelo okanye uxinzelelo.Kwenzeka xa umntu elahlekile okanye enamazinga aphantsi e-enzyme glucose-6-phosphate dehydrogenase.Le enzyme inceda iiseli ezibomvu zegazi zisebenze kakuhle.Iimpawu ngexesha le-hemolytic episode zingabandakanya umchamo omnyama, ukukhathala, ukuphaphatheka, ukubetha kwentliziyo ngokukhawuleza, ukuphefumula okufutshane, kunye nolusu oluphuzi (i-jaundice).Ukunqongophala kwe-G6PD kuzuzwa ngendlela ye-X edibeneyo kwaye iimpawu zixhaphake kakhulu kumadoda (ingakumbi ama-Afrika aseMelika kunye nalawo avela kwiindawo ezithile zase-Afrika, e-Asia, naseMeditera).Ibangelwa utshintsho lwemfuzo kwi-G6PD gene.
I-Glucose-6-Phophate Dehydrogenase (G-6-PDH) iqulethe iiyunithi ezimbini zobunzima obulinganayo bemolekyuli.Ulandelelwano lwe-amino acid ye-monomer ishicilelwe.G-6-PDH isetyenziswe kwiimvavanyo ze-nicotinamide adenine dinucleotide kunye ne-tissue pyridine. ii-nucleotides.I-G-6-PDH inokuphinda isebenze kwizisombululo ezenziwe nge-urea.
IGlucose 6-phosphate dehydrogenase yi-enzayim engundoqo yokulawula kwinyathelo lokuqala lendlela ye-pentose phosphate.I-G-6-P-DH i-oxidizes glucose-6-phosphate phambi kwe-NADP+ ukuvelisa i-6- phosphogluconate.I-Polyacrylamide gel electrophoresis, ukungcola komsebenzi, kunye ne-anti-yeast G-6-PDH i-antibody immunoblotting studies ibonise ukuba i-G-6-PDH yi-glycoprotein.
Ulwakhiwo lweMichiza
Umgaqo wokusabela
D-Glucose-6-phosphate + NAD+→D-Glucono-δ-lactone-6-phosphate + NADH+H+
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Umgubo omhlophe we-amorphous, u-lyophilized |
| Umsebenzi | ≥150U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| Ukunyibilika (10mg umgubo/ml) | Cacileyo |
| I-NADH/NADPH oxidase | ≤0.1% |
| I-Glutathione reductase | ≤0.001% |
| I-Phosphoglucose isomerase | ≤0.001% |
| I-Creatine phosphokinase | ≤0.001% |
| I-6-Phosphogluconate dehydrogenase | ≤0.01% |
| Myokinase | ≤0.01% |
| Hexokinase | ≤0.001% |
Ukuthutha kunye nokugcinwa
Ezothutho: I-Ambient
Ugcino :Gcina kwi-2-8°C
Uvavanyo kwakhona olucetyiswayoUbomi:Iminyaka emi-2
