DNase I (Rnase Free) (2u/ul)
Inombolo yekati: HC4007B
I-DNase I yi-endonuclease ekwazi ukwetyisa i-DNA enye okanye ephindwe kabini.Iyakwazi i-hydrolyze iibhondi ze-phosphodiester ukuvelisa i-mono-kunye ne-oligodeoxynucleotides equkethe iqela le-5'-phosphate kunye neqela le-3'-OH.Uluhlu lwe-pH olusebenzayo lwe-DNase I luyi-7-8.Umsebenzi we-DNase I uxhomekeke kwiCa2+kwaye inokuthi isebenze nge-ion zetsimbi ze-divalent ezifana ne-CO2, Mnu2+, Zn2+, njl. Phambi koMg2+, I-DNase ndiyakwazi ukucanda ngokungaqhelekanga nayiphi na indawo ye-DNA ephindwe kabini;Ngethuba ebukho bukaMn2+, DNase Ndiyakwazi ukucanda i-DNA ephindwe kabini kwindawo enye, yenze iziphelo ezinqabileyo okanye iziphelo ezincangathi kunye ne-1-2 nucleotides ephumayo.Ingasetyenziselwa ukusetyenzwa kweesampuli ezahlukeneyo ze-RNA.
Amacandelo
| Igama | 1KU | 5KU |
| I-Recombinant DNaseI (i-RNase-free) | 500 μL | 5 ×500 μL |
| I-DNase I Reaction Buffer (10×) | 1 ml | 5 × 1mL |
Iimeko zokugcina
Le mveliso kufuneka igcinwe kwi -25 ~ -15 ℃ iminyaka emi-2.Nceda uthintele ukunyibilika komkhenkce okuphindaphindiweyo.
Imiyalelo
Isetyenziswe ekukhutshweni kwe-DNA kwiisampuli ze-RNA ukuze kubhengezwe kuphela.
1. Nceda usebenzise i-RNase-free centrifuge tubes kunye neengcebiso zepipette ukulungiselela le nkqubo yokusabela ilandelayo:
| Amacandelo | Umthamo (μL) |
| I-DNase I Reaction Buffer (10×) | 1 |
| I-Recombinant DNasel (i-RNase-free) | 1 |
| I-RNA | X |
| RNase-free ddH2O | Ukuya kuthi ga kwi-10 |
2. Iimeko zokuphendula zimi ngolu hlobo lulandelayo: 37℃, emva kwe-15-30 mins, yongeza i-concentration yokugqibela ye-2.5 mM EDTA isisombululo kwaye udibanise kakuhle, emva koko u-65℃ ngemizuzu eyi-10.Ithemplate ecutshungulwayo ingasetyenziselwa i-RT-PCR okanye imifuniselo ye-RT-qPCR elandelayo, njl.
Amanqaku
1. I-DNase l inovelwano kwi-denaturation yomzimba;Xa udibanisa, uguqule ngobumnene umbhobho wokuvavanya kunyevuthulula kakuhle, ungashukumi ngamandla.
2. I-enzyme kufuneka igcinwe kwibhokisi yomkhenkce okanye kwibhafu yomkhenkce xa isetyenziswa, kwaye kufuneka igcinwe ku -20℃ ngokukhawuleza emva kokusetyenziswa.
3. Le mveliso isetyenziselwa uphando kuphela.
4. Nceda usebenzise iidyasi zelebhu kunye neeglavu ezilahlwayo, ukulungiselela ukhuseleko lwakho.
