I-M-MLV Reverse Transcriptase (iGlycerol simahla)
A lyophilizable Reverse Transcriptase.Ingafakwa kwitekhnoloji ye-lyophilization esezantsi ngelixa ugcina uguqulelo olukhulu lokukhuphela kunye nokuzinza.Le mveliso ayinazo izinto ezincedayo, nceda wongeze eyakho njengoko kufuneka.
Amacandelo
| Icandelo | HC2005A-01 (10,000U) | HC2005A-02 (40,000U) |
| UReverse Transcriptase (iGlycerol Mahala) (200U/μL) | 50 μL | 200 μL |
| 5 × Buffer | 200 μL | 800 μL |
Isicelo:
Isebenza kwinyathelo elinye le-RT-qPCR yokusabela.
Imeko yoGcino
Gcina kwi -30 ~ -15 ° C kunye nokuthutha kwi-≤0 ° C.
Inkcazelo yeyunithi
Iyunithi enye (U) ichazwa njengobungakanani be-enzyme edibanisa i-1 nmol ye-dTTP kwizinto ezingenayo i-asidi kwi-10mins kwi-37 ° C, kunye ne-Poly (rA) · Oligo (dT) njenge-template / primer.
Amanqaku
Isetyenziselwa uphando kuphela.Ayisetyenziswa kwiinkqubo zokuxilonga.
1.Nceda ugcine indawo yovavanyo icocekile;Nxiba iiglavu ezilahlwayo kunye nemaski;Sebenzisa i-RNase-free consumables efana ne-centrifuge tubes kunye neengcebiso ze-pipette.
2.Gcina i-RNA emkhenkceni ukunqanda ukuthotywa.
3.Iitemplates ezikumgangatho ophezulu we-RNA ziyacetyiswa ukuba zifezekise ukukhutshelwa umva kokusebenza okuphezulu.
