IGlucose Dehydrogenase (GDH)
Inkcazo
I-Glutamate Dehydrogenase (GDH) yi-enzyme ye-mitochondrial eyenza ukuguqulwa kwe-oxidative deamination ye-glutamate kwi-a-ketoglutarate kwaye isebenza njengekhonkco eliphambili phakathi kwe-anabolic kunye neendlela ze-catabolic.Kwizilwanyana ezanyisayo, i-GDH iphantsi kolawulo lwe-allosteric kwaye inomsebenzi ophezulu kwisibindi, izintso, ingqondo kunye ne-pancreas.Umsebenzi we-GDH kwi-serum ingasetyenziselwa ukwahlula phakathi kwezifo zesibindi ngenxa yokuvuvukala kwesibindi, okungabonisi umsebenzi ophakamileyo we-serum GDH, kunye nezifo ezibangela i-hepatocyte necrosis, ephumela kwi-serum GDH ephakamileyo.
Umsebenzi we-GDH ugqitywe luvavanyo lwe-enzyme oludityanisiweyo apho i-glutamate ityiwa yi-GDH evelisa i-NADH, esabela nge-probe evelisa i-colorimetric (450 nm) imveliso ngokuhambelana nomsebenzi we-GDH okhoyo.Iyunithi enye ye-GDH sisixa se-enzyme esiza kuvelisa i-1.0 mmole ye-NADH ngomzuzu kwi-pH 7.6 ku-37 °C.
Ulwakhiwo lweMichiza
Indlela yokusabela
I-D-Glucose + isamkeli → D-Glucono-1,5-lactone + i-lactone encitshisiweyo
Inkcazo
| Izinto zovavanyo | Iinkcukacha |
| Inkcazo | Umgubo omhlophe we-amorphous, u-lyophilized |
| Umsebenzi | ≥160U/mg |
| Ubunyulu(SDS-PAGE) | ≥90% |
| Ukunyibilika (10 mg umgubo/mL) | Cacileyo |
| Ukungcolisa ii-enzymes | |
| IGlucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Ukuthutha kunye nokugcinwa
Ezothutho: Iipaki zomkhenkce
Ugcino :Gcina kwi -25~-15 ° C (Ixesha elide), 2-8 ° C (ixesha elifutshane)
Uvavanyo kwakhona olucetyiswayoUbomi: Iinyanga ezili-18
